Kayayyaki

Bayani:
pachymic acid triterpenoid ne daga P. cocos.Pachymic acid yana hana hanyoyin siginar Akt da ERK.

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Abubuwan Halitta > > terpenoids da glycosides

Manufar:
Ak
ERK

A cikin Nazarin Vitro:
pachymic acid (PA) na iya hana tumorigenesis na gallbladder ciwon daji wanda ya shafi Akt da ERK hanyoyin sigina.Maganin Pachymic acid (PA) ya hana Rho a, Akt da hanyoyin ERK a cikin ƙwayoyin ciwon daji na gallbladder.Maganin Pachymic acid (PA) na iya rage tsarin PCNA, ICAM-1, RhoA, p-Akt da perk ta hanyar dogaro da kashi.Sa'o'i 12 bayan jiyya, 10 μ G / ml Poria acid (PA) ya hana ci gaban kwayar halitta a wani taro na 30 μ G / ml yana kara rage girman girma.An hana haɓakar tantanin halitta a cikin lokaci-da kuma dogaro da kashi.Bayan sa'o'i 48 na jiyya, ƙaddamarwa shine 10 μ g / ml, 20 μ G / ml da 30 μ Pachymic acid (PA) a g / ml ya hana ci gaban cell ta kusan 25%, 40% da 70%.Pachymic acid (PA) kuma yana hana haɓakar ƙwayoyin cutar kansar gallbladder a cikin dogaro da lokaci da dogaro da kashi [1].

A cikin Nazarin Vivo:
domin a kimanta aikin antitumor na pachymic acid (PA) a cikin vivo, an yi amfani da samfurin ciwon huhu na mutum nci-h23 tumor xenograft.Pachymic acid (PA) yana hana haɓakar ƙari sosai a allurai na 30 da 60 mg / kg na kwanaki 21 idan aka kwatanta da ƙungiyar kulawa [2].

Gwajin Kwayoyin Halitta:
An yi amfani da kit-8 (CCK-8) don kimanta tasirin yaduwa na pachymic acid (PA) akan ƙwayoyin GBC-SD.A takaice, bayan ƙayyadaddun magani, ƙara 10 zuwa kowane rami μ Lcck-8 bayani, kuma bayan shiryawa ɗaya, an auna abin sha a 450 nm ta amfani da mai karanta microplate [1].

Gwajin Dabbobi:
beraye [2] sun yi amfani da mice tsirara athymic mata masu shekaru 4-5.Kwayoyin nci-h23 masu girma da yawa (a 100 μ 5 a cikin LPBs × 106) an yi musu allurar subcutaneously cikin gefen dama na kowane linzamin kwamfuta.Tumor xenografts sun girma zuwa matsakaicin girman 100-200mm 3 kuma an ba da izini ga ƙungiyoyin jiyya daban-daban guda huɗu (6 mice a kowace ƙungiya): (a) sarrafa abin hawa (0.1% DMSO a cikin saline na al'ada);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg / kg;(Dd) PA 60mg/kg. An ba wa berayen allura ta intraperitoneal (IP) na tsawon makonni 3 (kwana 5 / mako).An auna girman ƙwayar ƙwayar cuta akan gatura biyu tare da taimakon vernier caliper, kuma an ƙididdige ƙarar ƙari (mm3).

Magana:
[1].Chen Y, et al.Pachymic acid yana hana tumorigenesis a cikin gallbladder carcinoma cell.Int J Clin Exp Med.2015 Oktoba 15; 8 (10): 17781-8.
[2].Ma J, et al.Pachymic acid yana haifar da apoptosis ta hanyar kunna ROS-dogara JNK da hanyoyin damuwa na ER a cikin ƙwayoyin kansar huhu.Cancer Cell Int.2015 ga Agusta 5;15:78.