Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
ʻIke Pono
ʻO Salvianolic acid B ka condensation o ʻekolu mole o Danshensu a me hoʻokahi mole o ka ʻakika caffeic.ʻO ia kekahi o nā waikawa salvianolic i aʻo ʻia.Loaʻa iā ia nā hopena pharmacological koʻikoʻi ma ka puʻuwai, ka lolo, ka ate, ka puʻupaʻa a me nā mea ʻē aʻe.Loaʻa i kēia huahana nā hopena o ka hoʻoikaika ʻana i ke kahe koko a me ka wehe ʻana i ke koko stasis, dredging meridian a me ka hoʻoulu ʻana i nā collaterals.Hoʻohana nui ʻia ia e mālama i ka maʻi ischemic i hoʻokumu ʻia e ke koko e kāohi ana i nā meridian, e like me ka helu ʻana o ka hapalua o ke kino a me nā lālā, nāwaliwali, ʻeha contracture, pau ʻole kaʻa, waha a me ka deflection o ka maka, etc.
Alias:salvianolic acid B, salvianolic acid B, salvianolic acid B
inoa Pelekania:salvianolic acid B
ʻAno molekala:c36h30o16
kaumaha molekala:718.62
Helu CAS:115939-25-8
ʻAno ʻike:HPLC ≥ 98%
Nā kikoʻī:10mg, 20mg, 100mg, 500mg, 1g (hiki ke hoʻopili ʻia e like me nā koi o ka mea kūʻai aku)
Hana a hoʻohana:Hoʻohana ʻia kēia huahana no ka hoʻoholo ʻana i ka ʻike.
ʻAno kino a me kemika
Nā waiwai:he pauda keʻokeʻo like ka huahana.
He ʻawaʻawa iki ka ʻono a me ka astringent, me ka waiwai hoʻoulu wai.Hiki ke hoʻoheheʻe ʻia i ka wai, ethanol a me ka methanol.
Hoʻokumu ʻia ka ʻakika Salvianolic B e ka condensation o 3 mau mole o ka salvianolic acid a me 1 mole o ka ʻakika caffeic.Loaʻa iā ia nā hui carboxyl ʻelua a aia ma ke ʻano o nā paʻakai like ʻole (K +, Ca2 +, Na +, NH4 +, etc.).I ke kaʻina hana o ka Decoction a me ka hoʻopaʻa ʻana, ua hydrolyzed kahi hapa liʻiliʻi o ka salvianolic acid B i ka waika oxalic poni a me ka salvianolic acid, a ʻo kahi hapa o ka salvianolic acid B e lilo i rosmarinic acid ma lalo o nā kūlana acidic;Hiki i ka Salvianolic acid A a me C ke tautomeric i ka hoʻonā.
Nā kikoʻī
＞5%，＞10%，＞50%，＞70%，＞90%，98%
Kaʻina Wehe
Ua ʻoki ʻia ka Radix Salviae Miltiorrhizae, hoʻokomo ʻia i loko o ka pahu hoʻoheheʻe, hoʻoinu ʻia me 8 mau manawa o ka nui o 0.01mol/l hydrochloric acid i ka pō, a laila hoʻopili ʻia me 14 mau manawa o ka nui o ka wai.Hoʻomaʻemaʻe ʻia ka hopena i unuhi ʻia e AB-8 macroporous resin column.ʻO ka mea mua, e hoʻoheheʻe me 0.01mol/l hydrochloric acid e wehe i nā mea haumia ʻole i hoʻopili ʻia, a laila elute me 25% ethanol e wehe i nā haumia polar loa.ʻO ka mea hope loa, e hoʻopololei i ka 40% ethanol eluent ma lalo o ke kaomi hoʻemi ʻia e hoʻihoʻi hou i ka ethanol a me ka maloʻo maloʻo no ka loaʻa ʻana o ka Salvia miltiorrhiza phenolic acid me ka maʻemaʻe ma mua o 80%.
E hoomaopopo
E lawe i 1g o ka huahana, wili iā ia, hoʻohui i 5ml o ka ethanol, hoʻoulu piha, kānana, lawe i kekahi mau kulu o ka kānana, kikoʻī ma ka pepa kānana, hoʻomaloʻo, nānā ma lalo o ke kukui ultraviolet (365nm), hōʻike i ka polū- 'āhinahina, e kau i ka pepa kānana i loko o ka 'ōmole solution ammonia concentrated ('a'ole e pili ana i ka ili wai), e lawe i waho ma hope o 20 minuke, e nana ma lalo o ke kukui ultraviolet (365nm), e hō'ike i ka 'ula'ula uliuli-'ōma'oma'o.
ʻakika:e lawe i ka wai hoʻonā ma lalo o ka mea o ka clarity, a me ka pH waiwai e 2.0 ~ 4.0 (appendix o Chinese Pharmacopoeia 1977 Edition).
Hoʻoholo maʻiʻo
Ua hoʻoholo ʻia e ka chromatography wai hana kiʻekiʻe (Appendix VI D, Volume I, Chinese Pharmacopoeia, 2000 EDITION).
Ua hoʻohana ʻia ʻo Octadecyl silane i hoʻopaʻa ʻia i ka silica gel i mea hoʻopiha i nā kūlana chromatographic a me ka hoʻāʻo ʻana i ka ʻōnaehana;ʻO Methanol acetonitrile formic acid wai (30:10:1:59) ʻo ia ka pae neʻe;ʻO 286 nm ka lōʻihi nalu.ʻAʻole emi ka helu o nā papa theoretical e like me ka salvianolic acid B peak ma mua o 2000.
ʻO ka hoʻomākaukau ʻana i ka haʻina haʻihaʻi e kaupaona pono i ka nui kūpono o ka salvianolic acid B reference solution a hoʻohui i ka wai e loaʻa ai ka 10% no ka 1ml μG solution.
E hoʻomākaukau i ka hopena hoʻāʻo e lawe ma kahi o 0.2g o ka huahana, e kaupaona pono iā ia, e hoʻokomo iā ia i loko o kahi ʻōmole anana 50ml, e hoʻohui i kahi nui kūpono o ka methanol, sonicate no 20 mau minuke, e hoʻomaʻalili, e hoʻohui i ka wai i ka unahi, e lulu maikaʻi, kānana. ia, e ana pono i 1ml o ka filtrate mau, e hookomo iloko o ka omole ana 25ml, e hookomo i ka wai i ka unahi, e lulu maikai.
Hoʻopili pololei ke ʻano hoʻoholo i ka 20% o ka hoʻonā mana a me 20% o ka hopena hoʻāʻo μ l.E hoʻokomo i loko o ka chromatograph wai no ka hoʻoholo ʻana.
ʻO ka maikaʻi o ka lāʻau lapaʻau
ʻO Salvianolic acid B ka condensation o ʻekolu mole o Danshensu a me hoʻokahi mole o ka ʻakika caffeic.ʻO ia kekahi o nā waikawa salvianolic i aʻo ʻia.Loaʻa iā ia nā hopena pharmacological koʻikoʻi ma ka puʻuwai, ka lolo, ka ate, ka puʻupaʻa a me nā mea ʻē aʻe.
Antioxidant
He hopena antioxidant ikaika ko Salvianolic acid B.Hōʻike nā hoʻokolohua i loko o vivo a me in vitro hiki i ka salvianolic acid B ke ʻohi i nā radical manuahi a pale i ka lipid peroxidation.ʻOi aku ka ikaika o kāna hana ma mua o ka huaora C, ka huaora E a me ka mannitol.ʻO ia kekahi o nā huahana kūlohelohe i ʻike ʻia me ka hopena antioxidant ikaika loa ʻO nā haʻawina Pharmacological e hōʻike ana i ka salvianolic acid no ka injection he hopena antioxidant maopopo, ke kāohi i ka platelet aggregation a me ka thrombosis, a hiki ke hoʻolōʻihi i ka manawa ola o nā holoholona ma lalo o hypoxia.Ua hōʻike ʻia nā hopena i hiki i ka salvianolic acid no ka injection (60 ~ 15mg / kg) ke hoʻomaikaʻi maikaʻi i ka deficit neurological i nā ʻiole me ka cerebral ischemia-reperfusion injury, hoʻomaikaʻi i ka maʻi maʻi a hoʻemi nui i ka wahi o ka cerebral infarction.He ʻokoʻa koʻikoʻi ma waena o nā kiʻekiʻe a me nā haʻahaʻa haʻahaʻa (60 a me 30mg / kg);Hiki i ka Salvianolic acid no ka injection ke hoʻomaikaʻi nui i ka pōʻino neurological i hoʻokumu ʻia e FeCl3 i hoʻokomo ʻia i ka cerebral ischemia i nā ʻiole ma 1, 2 a me 24 mau hola ma hope o ka lawelawe ʻana, i hōʻike ʻia i ka hoʻomaikaʻi ʻana i ka maʻi ʻeha a me ka hōʻemi ʻana o ka wahi cerebral infarction;ʻO ka Salvianolic acid 40 mg / kg no ka injection i hoʻopaʻa nui ʻia i ka hui ʻana o nā platelets rabbit i hoʻokomo ʻia e ADP, arachidonic acid a me collagen, a ʻo nā helu inhibition he 81,5%, 76,7% a me 68,9%.ʻO ka Salvianolic acid 60 a me 30mg / kg no ka injection i hoʻopaʻa nui i ka thrombosis i nā ʻiole;ʻO ka Salvianolic acid 60 a me 30mg / kg no ka injection i hoʻolōʻihi i ka manawa ola o nā ʻiole ma lalo o ka hypoxia.
Hoʻohana lāʻau lapaʻau
Loaʻa i kēia huahana nā hopena o ka hoʻoikaika ʻana i ke kahe koko a me ka wehe ʻana i ke koko stasis, dredging meridian a me ka hoʻoulu ʻana i nā collaterals.Hoʻohana nui ʻia ia e mālama i ka maʻi ischemic i hoʻokumu ʻia e ke koko e kāohi ana i nā meridian, e like me ka helu ʻana o ka hapalua o ke kino a me nā lālā, nāwaliwali, ʻeha contracture, pau ʻole kaʻa, waha a me ka deflection o ka maka, etc.
Halekuai
Ma kahi maloʻo a maloʻo.
Manawa Manawa
ʻElua makahiki.
Keʻano mālama
2-8 ° C, mālama ʻia ma kahi maloʻo a maloʻo a mamao mai ka mālamalama.
Nā mea e pono ai ka nānā
Pono e mālama ʻia ka huahana ma kahi haʻahaʻa haʻahaʻa.Inā lōʻihi ka ʻike ʻana i ka ea, e hoʻemi ʻia ka ʻike.
