Verbascoside CAS No. 61276-17-3
ʻIke Pono
[inoa]ʻO Mullein glycoside
[alias]ergosterol, Mullein
[ʻano]phenylpropanoid glycosides
[inoa Pelekania]acteoside;Verbascoside;Kusaginin
[kumu molekala]C29H36O15
[kaumaha molekula]624.59
[CAS No.]61276-17-3
Na Waiwai Kinohi
[nā waiwai]ʻO kēia huahana he pauka kristal keʻokeʻo nila
[pili pili]1.6g/cm3
[solubility]hiki ke hoʻoheheʻe maʻalahi i ka ethanol, methanol a me ka ethyl acetate.
Puna Hoʻoemi
ʻO kēia huahana ke kumu ʻiʻo maloʻo me nā lau scaly o Cistanche deserticola, kahi mea kanu o ka ʻohana liedang.
ʻAno hoʻāʻo
HPLC ≥ 98%
Nā kūlana Chromatographic: mobile phase methanol acetonitrile 1% acetic acid (15:10:75), kahe kahe 0.6 ml · min-1, kolamu wela 30 ℃, ʻike hawewe lōʻihi 334 nm (no ka ʻike wale nō)
Hana a hoʻohana
Hoʻohana ʻia kēia huahana no ka hoʻoholo ʻana i ka ʻike
Keʻano mālama
2-8 ° C, mālama ʻia mai ka mālamalama.
Bioactivity o Verbascoside
Haʻawina In Vitro:
Ma keʻano he mea hoʻokūkū PKC Inhibitor o ATP, loaʻa iā Verbascoside he IC50 o 25 μ M. Ua hōʻike ʻo Verbascoside i ka kis o 22 a me 28 e pili ana i ka ATP a me ka histone, kēlā me kēia μ M. Loaʻa i ka Verbascoside ka hana antitumor maikaʻi ma L-1210 mau mea me IC50 o 13 μM [1] 。 Verbascoside (5,10) μ M) Inhibition o 2,4-dinitrochlorobenzene (DNCB) - i ho'okomo 'ia T cell costimulatory kumu CD86 a me CD54, proinflammatory cytokines a me NF i thk-1 mau keena κ B ala ho'āla [2].
Ma nā haʻawina Vivo:
ʻO Verbascoside (1%) ka mea i hōʻemi i ka ulu ʻana o ka hana ʻoki āpau a me ke ʻano nui o nā ʻili ʻili i loko o ke kumu hoʻohālike ʻiole o 2,4-dinitrochlorobenzene (DNCB) - induced atopic dermatitis (AD).Hiki nō hoʻi iā Verbascoside ke ālai i ka TNF cytokine pro-inflammatory ma DNCB i hoʻokomo ʻia i nā ʻili ʻili- α， Hōʻike o IL-6 a me IL-4 mRNA [2].ʻAʻole i hoʻololi ʻo Verbascoside (50100 mg / kg, IP) i ka ʻeha ʻeha maʻamau i hoʻokumu ʻia e ka ʻeha compressive maʻi (CCI).ʻO Verbascoside(200 mg / kg, IP) i hōʻemi i ka maʻi maʻi i ka acetone i hoʻoulu ʻia i ke anuanu ma ka lā 3. Ua hoʻemi nui ʻo Verbascoside i nā loli ʻano pili me ka neuropathy.Eia kekahi, ua hoʻemi ʻo Verbascoside i ka Bax a hoʻonui i ka Bcl-2 i ka lā 3 [3].
ʻO ka hoʻokolohua kelepona:
lymphocytic mouse leukemia L1210 cell (ATCC, CCL 219) loaʻa 10% fetal bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ I loko o ka 24 well cluster plate of Dulbecco modified Eagle medium, 104 cell per well was sparsely lay/ Ml streptomycin sulfate a me Verbascoside (hoʻoheheʻe ʻia i DMSO).Ua nānā ʻia ka ulu ʻana ma ka helu ʻana i ka helu o nā cell i ka Coulter counter ma hope o 2 mau lā o ka incubation i loko o kahi ea humid (5% CO2 i ka ea) ma 37 ℃.Ua helu ʻia ka waiwai IC50 ma muli o ka laina regression linear i hoʻokumu ʻia no kēlā me kēia pūhui hoʻāʻo [1].
Hoʻokolohua holoholona:
I mea e hoʻoulu ai i ka dermatitis atopic (AD) - e like me nā hōʻailona, ua hoʻohana nā ʻiole [2] i ka 2,4-dinitrochlorobenzene (DNCB).I ka pōkole, ua wehe ʻia ka lauoho hope o nā ʻiole me nā ʻoki uila 2 mau lā ma mua o ka mālama ʻana i ka DNCB.E hoʻohana ʻia ka 200 μ L o 1% DNCB (ma ka acetone: ʻaila ʻoliva = 4:1) i ka ʻili i kahi ʻia i hope no ka ʻike.Hana ʻia nā hoʻouka hou ʻana ma ka pūnaewele hoʻokahi, 0.2% DNCB i kēlā me kēia 3 lā no kahi 2 pule.Ua māhele ʻia nā ʻiole i nā pūʻulu 4 (n = 6 i kēlā me kēia hui): (1) mālama ʻia ka kaʻa, (2) mālama ʻia ʻo DNCB wale nō, (3) 1% Verbascoside (acetone: ʻaila ʻoliva 4: 1) - mālama wale ʻia, a ( 4) DNCB + 1% hui mālama ʻia ʻo Verbascoside[2].
Kuhikuhi:
[1].Herbert JM, et al.Hoʻokaʻawale ʻia ʻo Verbascoside mai Lantana camara, kahi mea hoʻopale o ka protein kinase C. J Nat Prod.1991 Nov-Dek;54(6):1595-600.
[2].Li Y, et al.Hoʻopau ʻo Verbascoside i nā hōʻailona like me Atopic Dermatitis i nā ʻiole ma o kāna hopena anti-inflammatory ikaika.Int Arch Allergy Immunol.2018;175(4):220-230.
[3].Amin B, et al.ʻO ka hopena o Verbascoside i ka ʻeha Neuropathic i hoʻoulu ʻia e ka hōʻeha ʻeha ʻana i nā ʻiole.ʻO Phytother Res.2016 Jan;30(1):128-35.
