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Nqe lus piav qhia:isoorientin yog ib qho zoo COX-2 inhibitor nrog IC50 tus nqi ntawm 39 μ M.

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Lub hom phiaj: cox-2: 39 μ M (IC50)

Kev tshawb fawb hauv vitro:Isoorientin yog ib qho kev xaiv inhibitor ntawm cyclooxygenase-2 (COX-2) los ntawm tuber ntawm Pueraria tuberosa [1].PANC-1 thiab patu-8988 hlwb tau kho nrog Isoorientin (0,20,40,80 thiab 160 μ M) Loj hlob nyob rau hauv lub xub ntiag ntawm 24 teev thiab ntxiv cov tshuaj CCK8.Ntawm 20, 40, 80 thiab 160 μ Ntawm qhov concentration ntawm M, cell viability txo qis.Isoorientin (0,20,40,80 thiab 160) tau siv rau cov hlwb μ M rau PANC-1;0, 20, 40, 80160 thiab 320 μ M tau siv rau patu-8988) kab lis kev cai rau 24 teev, thiab kev qhia ntawm P tau soj ntsuam los ntawm Western blot - AMPK thiab AMPK.Kev qhia ntawm p-ampk tau nce ntxiv tom qab kev kho Isooritinin.Tom qab ntawd, hauv pawg shRNA, 80 μ M concentration txhawm rau txheeb xyuas cov nyhuv ntawm Isoorientin.Cov theem qhia ntawm AMPK thiab p-ampk hauv shRNA pawg tau qis dua li cov hauv cov tsiaj qus-hom PC hlwb (WT) thiab pab pawg kis nrog kev tswj tsis zoo lentivirus (NC) [2].

Hauv vivo kev tshawb fawb:Tsiaj txhu kho nrog Isoorientin ntawm 10 mg / kg thiab 20 mg / kg lub cev qhov hnyav tau txo qis hauv claw edema, nrog rau qhov nruab nrab ncov tuab ntawm 1.19 ± 0.05 mm thiab 1.08 ± 0.04 mm, ntsig txog.Qhov no tau qhia tias Isoorientin txo qis paw edema piv nrog rau pawg tswj hwm [3].

Kev sim ntawm tes:PANC-1 thiab patu-8988 hlwb raug inoculated ntawm 96 zoo daim hlau.Txhua lub qhov dej muaj ~ 5000 hlwb thiab 200 hlwb μ L nruab nrab muaj 10% FBS.Thaum cov hlwb hauv txhua qhov dej mus txog 70% qhov sib txuam, qhov nruab nrab tau hloov pauv thiab FBS dawb nruab nrab nrog ntau qhov sib txawv ntawm isoorientin ntxiv.Tom qab 24 teev, cov hlwb raug ntxuav ib zaug nrog PBS, kab lis kev cai nruab nrab uas muaj isoorientin raug muab pov tseg, thiab 100% tau ntxiv μ L FBS dawb nruab nrab thiab 10 μ L cell suav cov khoom siv 8 (CCK8) reagent.Cov hlwb raug incubated ntawm 37 ℃ rau lwm 1-2 teev, thiab qhov nqus ntawm txhua qhov dej tau kuaj pom ntawm 490 nm siv ELISA nyeem ntawv.Cell viability yog qhia raws li ntau yam kev hloov hauv absorbance [2].

Tsiaj kev sim:Nyob rau hauv cov ntaub ntawv ntawm paw edema qauv, nas [3] tau muab isoorientin los yog celecoxib intraperitoneally, thiab carrageenan tau ncaj qha txhaj rau hauv paw ib teev tom qab.Hauv cov qauv airbag, tag nrho cov kev kho mob nkag mus rau hauv lub hnab kab noj hniav ncaj qha nrog carrageenan.isoorientin tau txhaj tshuaj 3 teev ua ntej carrageenan tau txhaj rau hauv cov tshuaj ntsiav.Isoorientin thiab celecoxib tau muab rau cov nas.Cov kev daws teeb meem ntawm isoorientin (100 mg / ml) thiab celecoxib (100 mg / ml) tau npaj hauv DMSO thiab ntxiv diluted thaum kho.Cov tsiaj tau muab faib ua tsib pawg hauv qab no: kev tswj hwm (DMSO kho);Kho carrageenan (0.5 ml (1.5% (w / V) carrageenan hauv brine); Kho carrageenan + celecoxib (20mg / kg lub cev hnyav); Kho carrageenan + isoorientin (10 mg / kg lub cev hnyav); kho carrageenan + isoorientin (20mg / kg lub cev hnyav).

Siv:[1].Sumalatha M, et al.Isoorientin, ib qho Selective Inhibitor ntawm Cyclooxygenase-2 (COX-2) los ntawm Tubers ntawm Pueraria tuberosa.Nat Prod Commun.Peb Hlis 2015; 10(10): 1703-4.
[2].Ye T, et al.Isoorientin induces apoptosis, txo invasiveness, thiab downregulates VEGF secretion los ntawm activating AMPK signaling nyob rau hauv pancreatic cancer hlwb.Onco Targets Ther.2016 Dec 12; 9:7481-7492.
[3].Anilkumar K, et al.Kev soj ntsuam ntawm Anti-Inflammatory Properties ntawm Isoorientin cais los ntawm Tubers ntawm Pueraria tuberosa.Oxid Med Cell Longev.Xyoo 2017; 2017: 5498054.