Cov khoom

Nqe lus piav qhia:
pachymic acid yog triterpenoid los ntawm P. cocos.Pachymic acid inhibits Akt thiab ERK signaling txoj kev.

Pawg muaj feem:
kev tshawb fawb teb > > mob cancer
Cov khoom ntuj >> terpenoids thiab glycosides

Hom phiaj:
Akt
ERK

Kev tshawb fawb hauv Vitro:
pachymic acid (PA) tuaj yeem cuam tshuam cov qog nqaij hlav ntawm lub gallbladder cancer uas cuam tshuam nrog Akt thiab ERK signaling pathways.Pachymic acid (PA) kev kho mob cuam tshuam rau Rho a, Akt thiab ERK txoj hauv kev hauv lub gallbladder qog noj ntshav.Pachymic acid (PA) kev kho mob tuaj yeem txo qis PCNA, ICAM-1, RhoA, p-Akt thiab perk raws li koob tshuaj.12 teev tom qab kev kho mob, 10 μ G / ml Poria acid (PA) inhibited cell loj hlob ntawm ib tug concentration ntawm 30 μ G / ml ntxiv txo cell loj hlob.Cell loj hlob tau inhibited nyob rau hauv ib lub sij hawm - thiab koob tshuaj nyob ntawm seb.Tom qab 48 teev ntawm kev kho mob, qhov concentration yog 10 μ g / mL, 20 μ G / ml thiab 30 μ Pachymic acid (PA) ntawm g / ml inhibited cell loj hlob li ntawm 25%, 40% thiab 70%.Pachymic acid (PA) kuj inhibits kev loj hlob ntawm gallbladder cancer hlwb nyob rau hauv lub sij hawm-nyob thiab koob tshuaj-dependent yam [1].

Hauv Vivo Study:
Txhawm rau ntsuas qhov kev ua haujlwm antitumor ntawm pachymic acid (PA) hauv vivo, tib neeg lub ntsws cancer nci-h23 qog xenograft qauv tau siv.Pachymic acid (PA) cuam tshuam cov qog loj hlob ntawm koob tshuaj 30 thiab 60 mg / kg rau 21 hnub piv nrog rau pawg tswj hwm [2].

Kev sim Cell:
Cell suav cov khoom siv-8 (CCK-8) tau siv los ntsuas qhov kev tiv thaiv kev loj hlob ntawm pachymic acid (PA) ntawm GBC-SD hlwb.Hauv luv luv, tom qab kev kho mob tau teev tseg, ntxiv 10 rau txhua qhov μ Lcck-8 tov, thiab tom qab ib qho kev tsim tawm, qhov nqus tau ntsuas ntawm 450 nm siv tus nyeem ntawv microplate [1].

Kev sim tsiaj:
nas [2] siv poj niam athymic liab qab nas hnub nyoog 4-5 lub lis piam.Exponentially zus nci-h23 hlwb (ntawm 100 μ 5 hauv LPBs × 106) subcutaneously txhaj rau hauv txoj cai flank ntawm txhua tus nas.qog xenografts tau loj hlob mus rau qhov nruab nrab qhov loj ntawm 100-200mm 3 thiab random muab rau plaub pawg kho mob sib txawv (6 nas nyob rau hauv txhua pab pawg): (a) kev tswj tsheb (0.1% DMSO hauv ib txwm saline);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg / kg;(Dd) PA 60mg / kg. Cov nas tau txais pa los ntawm kev txhaj tshuaj intraperitoneal (IP) rau 3 lub lis piam (5 hnub / lub lis piam).Cov qog loj tau ntsuas ntawm ob axes nrog kev pab los ntawm vernier caliper, thiab cov qog ntim (mm3) raug xam.

Siv:
[1].Chen Y, et al.Pachymic acid inhibits tumorigenesis nyob rau hauv lub gallbladder carcinoma hlwb.Int J Clin Exp Med.2015 Kaum Ib Hlis 15; 8(10): 17781-8.
[2].Ma J, et al.Pachymic acid induces apoptosis ntawm activating ROS-dependent JNK thiab ER txoj kev ntxhov siab nyob rau hauv lub ntsws cancer hlwb.Cancer Cell Int.Peb 5, 2015; 15:78 PM