Ngwaahịa

Nkọwa:
pachymic acid bụ triterpenoid sitere na P. Cocos.Pachymic acid na-egbochi ụzọ akara Akt na ERK.

Ụdị emetụtara:
ubi nyocha > > kansa
Ngwaahịa eke > > terpenoids na glycosides

Ebumnuche:
Akt
ERK

N'ime Vitro Ọmụmụ:
pachymic acid (PA) nwere ike igbochi tumorigenesis nke ọrịa cancer gallbladder gụnyere ụzọ akara Akt na ERK.Ọgwụgwọ pachymic acid (PA) gbochiri ụzọ Rho a, Akt na ERK n'ime mkpụrụ ndụ kansa gallbladder.Ọgwụgwọ pachymic acid (PA) nwere ike belata PCNA, ICAM-1, RhoA, p-Akt na perk n'ụzọ dabere na dose.12 awa mgbe ọgwụgwọ gasịrị, 10 μ G / ml Poria acid (PA) gbochiri uto cell na mkpokọta 30 μG / ml na-ebelata uto cell.A gbochiri uto mkpụrụ ndụ n'otu oge yana dabere na dose.Mgbe awa 48 nke ọgwụgwọ gasịrị, ntinye uche bụ 10 μ g / ml, 20 μ G / ml na 30 μ Pachymic acid (PA) na g / ml na-egbochi uto cell site na 25%, 40% na 70%.Pachymic acid (PA) na-egbochikwa uto mkpụrụ ndụ cancer gallbladder na oge dabere na usoro dabere na dose [1].

Na Ọmụmụ Vivo:
iji nyochaa ọrụ antitumor nke pachymic acid (PA) na vivo, a na-eji ụdị ọrịa cancer akpa ume mmadụ nci-h23 tumor xenograft mee ihe.Pachymic acid (PA) na-egbochi uto tumor nke ukwuu na doses nke 30 na 60 mg / kg maka ụbọchị 21 ma e jiri ya tụnyere ndị na-achịkwa [2].

Nnwale cell:
A na-eji kit-8 (CCK-8) na-agụta sel iji nyochaa mmetụta mgbochi mgbasa nke pachymic acid (PA) na sel GBC-SD.Na nkenke, mgbe ọgwụgwọ akọwapụtara, tinye 10 na oghere ọ bụla μ Lcck-8 ngwọta, na mgbe otu incubation gasịrị, a na-atụ ihe ntinye ahụ na 450 nm site na iji microplate reader [1].

Nnwale anụmanụ:
oke [2] ji umu nwanyi athymic gba ọtọ gbara izu 4-5.Mkpụrụ ndụ nci-h23 toro ogologo oge (na 100 μ 5 na LPBs × 106) gbanyere anụ ahụ n'akụkụ aka nri nke òké ọ bụla.Tumor xenografts toro na nkezi nke 100-200mm 3 ma kenye ya n'usoro anọ dị iche iche na-agwọ ọrịa (6 ụmụ oke na otu ọ bụla): (a) njikwa ụgbọ ala (0.1% DMSO na saline nkịtị);(b) Pachymic acid (PA) 10 mg / n'arọ;(c) PA 30 mg / n'arọ;(Dd) PA 60mg / kg. A na-enye ụmụ oke pa site na intraperitoneal (IP) maka izu atọ (ụbọchị 5 / izu).A tụrụ nha etuto ahụ na anyụike abụọ site n'enyemaka vernier caliper, wee gbakọọ olu etuto ahụ (mm3).

Ntụaka:
[1].Chen Y, et al.Pachymic acid na-egbochi tumorigenesis na mkpụrụ ndụ carcinoma gallbladder.Int J Clin Exp Med.2015 Ọkt 15;8 (10): 17781-8 .
[2].Ma J, et al.Pachymic acid na-ebute apoptosis site na ịgbalite ROS-based JNK na ER ụzọ nrụgide n'ime mkpụrụ ndụ cancer akpa ume.Ọrịa Cancer Cell Int.2015 Ọgọst 5;15:78 .