Ngwaahịa

Typhonine glycoside

Puhuang bụ pollen akpọnwụ nke Typha angustifolia L., Typha Orientalis Presl ma ọ bụ osisi ndị yiri ya na Typhaceae.Ọdịnaya nke isorhamnetin-3-o-neohesperidin na Chinese Pharmacopoeia (Mbipụta 2005) ka HPLC kpebisiri ike.Ụzọ ndị bụ isi maka ikpebi isorhamnetin-3-o-neohesperidin na Typhae na nkwadebe ya bụ.

HPLC.N'okpuru mkpebi ọdịnaya nke Typha na Chinese Pharmacopoeia (Mbipụta 2005):

Ọnọdụ Chromatographic na nyocha ngwa ngwa sistemu: octadecyl silane bonded silica gel as filler;A na-eji mmiri Acetonitrile (15:85) mee ihe dị ka usoro mkpanaka;Ogologo obosara nke nchọpụta bụ 254 nm.Okpomọkụ kọlụm 40 ℃.Ọnụ ọgụgụ nke efere usoro ihe ọmụma agaghị abụ ihe na-erughị 1000 dịka ọnụ ọgụgụ kasị elu nke isorhamnetin-3-o-neohesperidin si dị.

Nkwadebe nke ngwọta nnwale: were ihe dị ka 0.5g nke ngwaahịa a, tụọ ya nke ọma, tinye ya na karama nha 50ml, tinye 45ml nke methanol, ọgwụgwọ ultrasonic (ike 250W, ugboro 20KHz) maka nkeji 30, mee ka ọ dị jụụ, tinye methanol na mmiri. ọnụ ọgụgụ, maa jijiji nke ọma, nyochaa ya, ma were nzacha na-aga n'ihu

Mkpebi nke isorhamnetin-3-o-neohesperidin na Puhuang na nkwadebe ya site na HPLC.Sistemụ mkpanaka mkpanaka izugbe bụ mmiri acetonitrile:

① Hp1050 chromatograph mmiri mmiri dị elu;Kọlụm Chromatographic C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);Usoro mkpanaka: mmiri acetonitrile (15:85);Ogologo oge nchọpụta: 254nm;Okpokoro griin haus okpomọkụ.

② Igwe mmiri chromatograph dị elu bụ usoro hp1050 nke ụlọ ọrụ Hewlett Packard dị na United States;Chromatographic kọlụm bondclone 10 C18 (3.9mm) × 300mm); Usoro mkpanaaka: mmiri acetonitrile (80:20);ogologo oge nchọpụta 254 nm, bandwit 4 nm;Ntụaka wavelength 550 nm, bandwit 100 nm;attenuation 8.

Fu Daxu et al.Kpebie ọdịnaya nke typhoniflorin na isorhamnetin-3-o-neohesperidin na mkpokọta flavone nke Typha sitere na HPLC na 1100 chromatograph.Kọlụm chromatographic bụ eklips XdB C18 (250mm × 4.6mm, 5 μ m) ； Usoro mkpanaka: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15: 78: 7; 15min: 23: 70: 7);Ogologo oge nchọpụta bụ 248 nm.Nkwadebe nke mkpokọta flavonoids wepụ nke Typha: 50g nke Typha e amịpụtara na 60% ethanol (500ml) × 2), 1H oge ọ bụla, na wepụ e lekwasịrị na 40 ℃, wepụ na agbaze na 600ml mmiri na centrifuged.A na-agbaze ihe fọdụrụ na 300ml mmiri na centrifud.Gwakọta ngwọta centrifugal, dozie olu na 1000ml na mmiri, gbanye ya na kọlụm nke resin macroporous (150ml macroporous resin dry weight 75g) na 1.5 ugboro nke ọgwụ ọjọọ, elute na 600ml mmiri na 450ml nke 20% ethanol iji wepụ. adịghị ọcha, wee elute na 600ml nke 50% ethanol, na eluent na-enweta site agụụ ihicha na 40 ℃.

Yang Yonghua et al.Ejiri electrophoresis capillary capillary arụ ọrụ dị elu yana chromatography mmiri mmiri dị elu iji chọpụta ọdịnaya nke isorhamnetin 232o2, neohesperidin na typhoside na Puhuang.Hpce-3d elu arụmọrụ capillary electrophoresis ngwá (Hewlett Packard, USA), quartz capillary kọlụm 50 μ m × 56cm (HP), lc-6a elu arụmọrụ chromatograph mmiri (Shimadzu ụlọ ọrụ nke Japan), C18 kọlụm 5mm × 200mm, urughuru size 5 μ M (ụlọ ọrụ Dalian Yite).HPCE ọnọdụ: ihe nchekwa 0.02mol/l borax-0.05mol/l 10% acetonitrile nke sodium dodecyl sulfate (SDS), voltaji 20kV, kọlụm okpomọkụ 30 ℃, nchọpụta wavelength 270nm, injection olu 30KPa · s.Ọnọdụ HPLC: usoro mkpanaka bụ mmiri acetonitrile (15:85), okpomoku kọlụm bụ 25 ℃, na ogologo oge nchọpụta bụ 254 nm.

Ihe ntụaka Typhaneoside

[aha]typhonioside [1]

[CAS Mba.]104472-68-6

[usoro nchọpụta]HPLC ≥ 98%

[Nkọwapụta]20mg 50mg 100mg 500mg 1g (nwere ike ngwugwu dị ka ahịa chọrọ)

[ihe onwunwe]amorphous ntụ ntụ