Verbascoside CAS Nọmba 61276-17-3
Ozi dị mkpa
[aha]Mullein glycoside
[utu aha]Mullein, ergosterol
[otu]phenylpropanoid glycosides
[Aha bekee]mmetụta nke acteoside;Verbascoside;Kusaginin
[usoro ihe omimi]C29H36O15
[molecular arọ]624.59
[CAS Mba.]61276-17-3
Njirimara physicochemical
[ihe onwunwe]ngwaahịa a bụ ọcha agịga kristal ntụ ntụ
[njupụta njupụta]1.6g / cm3
[solubility]na-agbaze ngwa ngwa na ethanol, methanol na ethyl acetate.
Isi mmalite mmịpụta
Ngwaahịa a bụ azuokokoosisi anụ ahụ kpọrọ nkụ nke nwere akwụkwọ mkpọnwụ nke Cistanche deserticola, osisi nke ezinụlọ liedang.
Usoro ule
HPLC ≥ 98%
Ọnọdụ chromatographic: usoro mkpanaka methanol acetonitrile 1% acetic acid (15:10:75), ọnụego eruba 0.6 ml · min-1, kọlụm okpomoku 30 ℃, nmpụta ogologo 334 nm (maka naanị ntụaka)
Ọrụ na ojiji
A na-eji ngwaahịa a maka ikpebi ọdịnaya
Usoro nchekwa
2-8 Celsius C, echekwara ya na ìhè.
Bioactivity nke Verbascoside
N'ime Vitro Ọmụmụ:
Dị ka asọmpi PKC Inhibitor nke ATP, Verbascoside nwere IC50 nke 25 μ M. Verbascoside gosipụtara kis nke 22 na 28 metụtara ATP na histone, n'otu n'otu μ M. Verbascoside (5,10) μ M) Mgbochi nke 2,4-dinitrochlorobenzene (DNCB) - ihe kpatara T cell costimulatory ihe CD86 na CD54, cytokines proinflammatory na NF na thk-1 sel κ B ụzọ ịgbalite [2].
Na ọmụmụ Vivo:
Verbascoside (1%) belatara omume nke ịkpachapụ anya n'ozuzu yana oke ọnya anụ ahụ na ụdị òké nke 2,4-dinitrochlorobenzene (DNCB) - atopic dermatitis (AD).Verbascoside nwekwara ike igbochi pro-inflammatory cytokine TNF na ọnya akpụkpọ anụ DNCB kpatara - α, Nkwupụta nke IL-6 na IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) agbanweghị ihe mgbu na-adịghị ahụkebe oyi nke mmerụ ahụ na-adịghị ala ala (CCI) kpatara.Verbascoside (200 mg / kg, IP) na-ebelata ihe nfụkasị na oyi na-atụ acetone n'ụbọchị 3. Verbascoside na-ebelatakwa nke ukwuu mgbanwe omume metụtara neuropathy.Na mgbakwunye, Verbascoside belatara Bax wee mụbaa Bcl-2 na ụbọchị 3 [3].
Nnwale cell:
lymphocytic mouse leukemia L1210 cell (ATCC, CCL 219) nwere 10% nwa ebu n'afọ serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ N'ime 24 ọma ụyọkọ nke Dulbecco gbanwetụrụ ugo ọkara, 104 sel kwa nke ọma nwere spain / rse. Ml streptomycin sulfate na Verbascoside (gbasara na DMSO).A na-enyocha uto site n'ịgụ ọnụ ọgụgụ mkpụrụ ndụ dị na counter Coulter ka ụbọchị 2 nke incubation gasịrị na ikuku iru mmiri (5% CO2 na ikuku) na 37 ℃.A gbakọrọ uru IC50 dabere na ahịrị nkwụghachi azụ nke edobere maka ogige ule ọ bụla [1].
Nnwale anụmanụ:
Iji kpalite atopic dermatitis (AD) - dị ka akara ngosi, oke [2] jiri 2,4-dinitrochlorobenzene (DNCB).Na nkenke, ejiri mkpa eletrọnịkị wepụ ntutu isi nke ụmụ oke ụbọchị 2 tupu ọgwụgwọ DNCB.A ga-etinye 200 μ L nke 1% DNCB (na acetone: mmanụ oliv = 4: 1) na akpụ akpụ azụ akpụkpọ maka nghọta.Emere mwakpo ugboro ugboro n'otu saịtị ahụ, 0.2% DNCB kwa ụbọchị 3 maka ihe dịka izu abụọ.E kewara oke oke n'ime otu 4 (n = 6 na otu ọ bụla): (1) njikwa ụgbọ ala, (2) DNCB na-emeso naanị, (3) 1% Verbascoside (acetone: mmanụ oliv 4: 1) - ọgwụgwọ naanị, na ( 4) DNCB + 1% Verbascoside mesoo otu[2].
Ntụaka:
[1].Herbert JM, et al.Verbascoside dịpụrụ adịpụ na Lantana camara, onye na-egbochi protein kinase C.J Nat Prod.1991 Nov-Dec;54(6):1595-600.
[2].Li Y, et al.Verbascoside na-ebelata Atopic Dermatitis-Dị ka Mgbaàmà na ụmụ oke site na Mmetụta Mgbochi Ọrịa Na-akpata.Int Arch Allergy Immunol.2018; 175 (4): 220-230 .
[3].Amin B, et al.Mmetụta nke Verbascoside na Neuropathic Pain Na-akpata site na mmerụ ahụ na-adịghị ala ala na oke.Phytother Res.2016 Jan; 30 (1): 128-35 .
