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Description: eochlorogenic acid is a natural polyphenol compound found in dried fruits and other plants. Neochlorogenic acid inhibits TNF- α And IL-1 β Produce. Neochlorogenic acid inhibited the expression of iNOS and COX-2 proteins. Neochlorogenic acid also inhibits phosphorylated NF- κ B p65 and p38 MAPK activation.

Related Categories: signal pathway > > immunity and inflammation

> > interleukin related

signaling pathway > > apoptosis > > TNF receptor

natural Products > > benzoic acids

Research Field > > inflammation / immunity

Target:p65

IL-1β

TNF-α

COX-2

In Vitro Studies: neochlorogenic acid (NCA) inhibits the expression of iNOS and COX-2 proteins and proinflammatory cytokines (such as TNF) in BV2 microglia- α And IL-1 β） The production showed that lipopolysaccharide (LPS) - induced no production decreased. In addition, phosphorylated p38 MAPK and NF- κ Bp65 was also inhibited by neochloric acid in activated microglia. LPS induced elevated iNOS and COX-2 levels in BV2 cells, but with 50 and 100 μ This increase was significantly inhibited after m-hypochlorous acid treatment [1].
Cell Experiment: mouse BV2 microglia were maintained in DMEM, supplemented with 5% FBS and 1% antibiotic antifungal agent at 37 ℃ in a humid incubator containing 5% CO 2. Neochlorogenic acid and dexamethasone as positive controls were dissolved in DMSO until the final concentration of the stock solution was 10 mm. Treatment with LPS and / or neochlorogenic acid was performed under serum-free conditions. The effect of neochlorogenic acid on cell viability of BV2 microglia stimulated by lipopolysaccharide (LPS) was measured. With or without LPS (4) μ G / ml) and neochlorogenic acid (10, 50 and 100 μ M) Cells were treated for 24 hours. Dexamethasone (10) μ M) For positive control. Cell viability was confirmed by MTT assay. Remove the medium from the well, add MTT, and then incubate the sample at 37 ℃ for 3 hours. Methyl crystals were dissolved by adding DMSO, and the absorbance value was measured at 540 nm using an enzyme labeling instrument [1].
References: [1] Kim M, et al. Neochlorogenic Acid Inhibits Lipopolysaccharide-Induced Activation and Pro-inflammatory Responses in BV2 Microglial Cells. Neurochem Res. 2015 Sep; 40(9):1792-8.