Zogulitsa

Dzina la Chingerezi:tanshinone IIA
Dzina lachi China:tanino |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA biological ntchito

Kufotokozera:
Tanshinone IIA (Tan IIA) ndi imodzi mwazolemba zazikulu zosungunuka zamafuta mumizu ya muzu wofiira Salvia miltiorrhiza.Tanshinone IIA imatha kuletsa angiogenesis poyang'ana gawo la protein kinase la VEGF / VEGFR2.

Magawo oyenera:
Kafukufuku > > matenda a mtima
Zachilengedwe > Quinones

Zolinga:
VEGF/VEGFR2[1]

Phunziro la In Vitro:Zotsatira za antitumor za tanshinone IIA zimaphatikizapo kuletsa kuchulukana kwa maselo otupa, kusokoneza ma cell a chotupa, kulimbikitsa chotupa cell apoptosis ndikuletsa kuukira kwa cell ndi metastasis.Tanshinone IIA anali ndi odana proliferative zotsatira pa maselo A549: IC50 wa tanshinone IIA pambuyo 24, 48 ndi 72 maola anali 145.3, 30.95 ndi 11.49, motero μ M. CCK-8 assay anagwiritsidwa ntchito kuwunika tanshinone IIA μ-80 μ-25. The proliferative ntchito ya ankachitira A549 maselo pa 24, 48 ndi 72 hours, motero.Zotsatira za CCK-8 zinasonyeza kuti tanshinone IIA ikhoza kulepheretsa kwambiri kufalikira kwa maselo a A549 modalira mlingo komanso nthawi.Kuchuluka kwa apoptosis ndi kulepheretsa kukula kwa maselo a maselo a A549 kunawonedwa patatha maola 48 mutalandira mankhwala osokoneza bongo (kuchuluka komwe kunagwiritsidwa ntchito kunali pafupifupi IC50 mtengo: tanshinone iia31 pa A549) μ M) . μ M), pansi kuwongolera kufotokoza kwa VEGF ndi VEGFR2 mapuloteni mu gulu la mankhwala ndi vekitala [1].Tanshinone IIA ndi imodzi mwa zigawo zambiri za Salvia miltiorrhiza mizu, zomwe zingateteze maselo a H9c2 ku apoptosis.Maselo a H9c2 othandizidwa ndi tanshinone IIA oletsa angiotensin II adapangitsa apoptosis mwa kuwongolera mafotokozedwe a PTEN (phosphatase ndi tensin homologue).PTEN ndi chotupa chopondereza chomwe chimagwira ntchito yayikulu mu apoptosis.Tanshinone IIA imalepheretsa angiotensin II (AngII) - kuchititsa apoptosis mwa kuwongolera mafotokozedwe a phosphatase ndi tensin homologue (PTEN) [2].Tanshinone IIA imachepetsa kufotokoza kwa mapuloteni a EGFR, ndipo IGFR imatseka njira ya PI3K / Akt / mTOR m'maselo a khansa ya m'mimba ya AGS [3].

Kuyesa Maselo:Maselo a A549 adawerengedwa mu gawo la logarithmic ndi ma cell 6000 (voliyumu ya 90 μ L) mu mbale ya 96.Will 10 μ L osiyana ndende tanshinone IIA (woipa woipa 80,60,40,30,20,15,10,5 ndi 2.5 μ M) Ndi ADM (womaliza ndende 8,4,2,1,0.5 ndi 0,25 μ M ) Anawonjezeredwa ku gulu la mankhwala, pamene gulu lolamulira loipa (gulu lonyamulira) linangowonjezera 10 μ Ldmso kapena saline yachibadwa popanda tanshinone IIA kapena Adm. Sakanizani maselo ndi CCK-8 reagent (100 μ L / ml sing'anga) kwa. maola ena a 2, ndipo kuyamwa kunawerengedwa pa 450 nm pogwiritsa ntchito owerenga microplate.Mlingo woletsa kuchuluka kwa ma cell kumawerengedwa motsatira njira iyi: kuchuluka koletsa kuchuluka (%) = 1 - [(A1-A4) / (A2-A3)] × 100, pomwe A1 ndi mtengo wa OD wa gulu loyesera mankhwala, A2 ndi mtengo wa OD wa gulu lolamulira lopanda kanthu, A3 ndi mtengo wa OD wa RPMI1640 sing'anga wopanda maselo, ndipo A4 ndi mtengo wa OD wa mankhwala omwe ali ndi chiwerengero chofanana ndi A1 koma opanda maselo.Mtengo wa IC50 udawerengedwa ndi kusanthula kosasinthika kosagwirizana ndi pulogalamu ya graphpad prism [1], yomwe imayimira kuchuluka kwa mankhwala omwe akuwonetsa 50% kuletsa kukula kwa maselo.

Zolozera:[1].Xie J, ndi al.Mphamvu ya antitumor ya tanshinone IIA pa anti-proliferation ndikuchepetsa mawu a VEGF/VEGFR2 pamtundu wa cell womwe si wang'ono wam'mapapo wamtundu wa A549.Acta Pharm Sin B. 2015 Nov;5(6):554-63.
[2].Zhang Z, ndi al.Tanshinone IIA imaletsa apoptosis mu myocardium mwa kuchititsa microRNA-152-3p kufotokoza ndipo potero kuchepetsa PTEN.Ndine J Transl Res.2016 Jul 15;8(7):3124-32.
[3].Su CC, ndi al.Tanshinone IIA imachepetsa kufotokoza kwa mapuloteni a EGFR, ndi IGFR kutsekereza njira ya PI3K/Akt/mTOR m'maselo a gastric carcinoma AGS onse mu vitro ndi mu vivo.Oncol Rep. 2016 Aug;36(2):1173-9.