Isoorientin;Homoorientin CAS No 4261-42-1
Amakuru y'ingenzi
Izina ry'igishinwa: isolysine
Izina ry'icyongereza: isoorientin
Icyongereza bita: homoorientin;(1S) -1,5-anhydro-1- [2-
CAS No.: 4261-42-1
Inzira ya molekulari: C21H20O11
Uburemere bwa molekuline: 448.3769
Ibyiza bya fiziki
Isuku: hejuru ya 99%, uburyo bwo kumenya: HPLC.
Ubucucike: 1.759g / cm3
Ingingo yo guteka: 856.7 ° C kuri 760 mmHg
Ingingo yerekana: 303.2 ° C.
Umuvuduko wamazi: 2.9e-31mmhg kuri 25 ° C.
Igikorwa cyibinyabuzima cya Isoorientin
Ibisobanuro:isoorientin ni inhibitori ya COX-2 ifite agaciro ka IC50 ifite 39 μ M。
Ibyiciro bijyanye:
Umwanya wubushakashatsi >> Kanseri Ibicuruzwa Kamere >> flavonoide
Umwanya wubushakashatsi >> gutwika / ubudahangarwa
Intego: cox-2: 39 μ M (IC50)
Mu bushakashatsi bwa vitro:Isoorientin ni inhibitori ya cyclooxygenase-2 (COX-2) iva mu kirayi cya Pueraria tuberosa [1].PANC-1 na patu-8988 selile zavuwe hamwe na Isoorientin (0,20,40,80 na 160 μ M) Gukura imbere yamasaha 24 hanyuma wongereho CCK8.Kuri 20, 40, 80 na 160 μ Mugihe cya M, imbaraga za selile zagabanutse cyane.Isoorientin (0,20,40,80 na 160) yakoreshejwe muri selile μ M kuri PANC-1;0, 20, 40, 80160 na 320 μ M yakoreshejwe mumico ya patu-8988) mumasaha 24, kandi imvugo ya P yasuzumwe na blot yo muburengerazuba - AMPK na AMPK.Imvugo ya p-ampk yiyongereye nyuma yo kuvura Isoorientin.Hanyuma, mumatsinda ya shRNA, 80 μ M kwibanda kugirango tumenye ingaruka za Isoorientin.Urwego rwo kwerekana imvugo ya AMPK na p-ampk mu itsinda rya shRNA rwari hasi cyane ugereranije no mu ngirabuzimafatizo zo mu bwoko bwa PC (WT) kandi itsinda ryandujwe na lentivirus mbi (NC) [2].
Mu bushakashatsi bwa vivo:Amatungo yavuwe na Isoorientin kuri 10 mg / kg na 20 mg / kg ibiro byumubiri byagabanutse cyane muburyo bwo kugabanuka kwinzara, hamwe nuburebure buri hejuru ya 1,19 ± 0,05 mm na 1.08 ± 0.04 mm.Ibi byerekanaga ko Isoorientin yagabanije cyane urwungano ngogozi ugereranije nitsinda rishinzwe kugenzura [3].
Ubushakashatsi bwakagari:PANC-1 na patu-8988 selile zatewe ku byapa 96.Buri riba ririmo selile ~ 5000 na selile 200 μ L igizwe na 10% FBS.Iyo selile muri buri riba zigeze kuri 70% ihuriro, uburyo bwarahinduwe kandi FBS yubusa hamwe nubushakashatsi butandukanye bwa isoorientin.Nyuma yamasaha 24, selile zogejwe rimwe hamwe na PBS, umuco wumuco urimo isoorientin warajugunywe, 100% hiyongeraho μ L FBS yubusa hamwe na 10 μ L kubara selile 8 (CCK8) reagent.Ingirabuzimafatizo zashyizwe kuri 37 ℃ mu yandi masaha 1-2, kandi kwinjiza buri riba byagaragaye kuri 490 nm ukoresheje umusomyi wa ELISA.Ubushobozi bwakagari bugaragazwa nkimpinduka nyinshi mukwinjira [2].
Ubushakashatsi ku nyamaswa:kubijyanye na paw edema yerekana, imbeba [3] zahawe isoorientin cyangwa celecoxib intraperitoneally, hanyuma carrageenan yinjizwa mumatako nyuma yisaha imwe.Muburyo bwo mu kirere, uburyo bwose bwo kuvura bwinjira mu mufuka wuzuye na karrageenan.isoorientin yatewe inshinge amasaha 3 mbere yuko carrageenan yinjizwa muri capsule.Isoorientin na celecoxib byahawe imbeba.Ibisubizo bya stock ya isoorientin (100 mg / ml) na celecoxib (100 mg / ml) byateguwe muri DMSO hanyuma bikomeza kuvangwa mugihe cyo kuvura.Inyamaswa zagabanyijwemo amatsinda atanu akurikira: kugenzura (DMSO ivurwa);Carrageenan ivuwe (0,5 ml (1.5% (w / V) karrageenan muri brine); Carrageenan + celecoxib (20mg / kg uburemere bwumubiri); kg uburemere bw'umubiri).
Reba:[1].Sumalatha M, n'abandi.Isoorientin, Inhibitori Yatoranijwe ya Cyclooxygenase-2 (COX-2) iva Mubijumba bya Pueraria tuberosa.Nat Prod Commun.2015 Ukwakira; 10 (10): 1703-4.
[2].Yego T, n'abandi.Isoorientin itera apoptose, igabanya ibitero, kandi igabanya ururenda rwa VEGF ikora ibimenyetso bya AMPK mu ngirabuzimafatizo za kanseri yandura.Intego za Onco.2016 Ukuboza 12; 9: 7481-7492.
[3].Anilkumar K, n'abandi.Isuzuma rya Anti-Inflammatory Ibintu bya Isoorientin Yitaruye Ibijumba bya Pueraria tuberosa.Oxid Med Cell Longev.2017; 2017: 5498054.
