Ibicuruzwa

Izina ry'icyongereza:tanshinone IIA
Abashinwa Alias:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA ibikorwa byibinyabuzima

Ibisobanuro:
Tanshinone IIA (Tan IIA) nimwe mubintu nyamukuru bivamo ibinure mumizi yumuzi wumutuku Saliviya miltiorrhiza.Tanshinone IIA irashobora kubuza angiogenezezi yibanda kuri protein kinase domaine ya VEGF / VEGFR2.

Ibyiciro bijyanye:
Urwego rwubushakashatsi >> Indwara yumutima
Ibicuruzwa Kamere >> Quinone

Intego:
VEGF / VEGFR2 [1]

Mu Kwiga Vitro:Ingaruka za antitumor ziterwa na tanshinone IIA zirimo kubuza ikwirakwizwa ry'uturemangingo tw’ibibyimba, guhungabanya uruziga rw'ibibyimba, guteza imbere kanseri y'ibibyimba no kubuza gutera kanseri y'ibibyimba na metastasis.Tanshinone IIA yagize ingaruka zo kurwanya selile A549: IC50 ya tanshinone IIA nyuma yamasaha 24, 48 na 72 yari 145.3, 30.95 na 11.49, μ M。 CCK-8 yakoreshejwe mu gusuzuma tanshinone IIA (2.5-80 μ M) Igikorwa cyo gukwirakwiza ingirabuzimafatizo A549 zavuwe ku masaha 24, 48 na 72.Ibisubizo bya CCK-8 byerekanaga ko tanshinone IIA ishobora kubuza cyane ikwirakwizwa rya selile A549 muburyo bukabije kandi bushingiye ku gihe.Apoptose ikomeye no gukura kwakagari kwingirabuzimafatizo za A549 byagaragaye nyuma yamasaha 48 nyuma yo kuvura ibiyobyabwenge (intumbero yakoreshejwe yari ifite agaciro ka IC50: tanshinone iia31 kuri A549) μ M） bl Guhindura iburengerazuba byagaragaje guhura na tanshinone IIA (31) muri selile A549 mumasaha 48 μ M), hepfo igenga imvugo ya poroteyine ya VEGF na VEGFR2 mu itsinda rivura ibiyobyabwenge na vector [1].Tanshinone IIA ni kimwe mu bintu byinshi bigize umuzi wa Saliviya miltiorrhiza, ushobora kurinda selile H9c2 kwirinda apoptose.Ingirabuzimafatizo za H9c2 zavuwe na tanshinone IIA yabujije angiotensin II yateje apoptose mu kugabanya imiterere ya PTEN (fosifata na homologue ya tensin).PTEN ni suppressor yibibyimba igira uruhare runini muri apoptose.Tanshinone IIA ibuza angiotensin II (AngII) - iterwa na apoptose mu kugabanya imvugo ya fosifata na tensin homologue (PTEN) [2].Tanshinone IIA igabanya poroteyine ya EGFR, kandi IGFR ihagarika inzira ya PI3K / Akt / mTOR muri kanseri yo mu gifu ya selile AGS [3].

Ubushakashatsi bwakagari:A549 selile zabazwe mugice cya logarithmic na selile 6000 (90 μ L ingano) muri plaque 96.Uzaba 10 μ L ubunini bwa tanshinone IIA (kwibanda kwanyuma 80,60,40,30,20,15,10,5 na 2.5 μ M) Na ADM (kwibanda kwanyuma 8,4,2,1,0.5 na 0.25 μ M ) Yongewe mumatsinda yibiyobyabwenge, mugihe itsinda rishinzwe kugenzura nabi (itsinda ryabatwara) ryongeweho 10 μ Ldmso cyangwa saline isanzwe idafite tanshinone IIA cyangwa Adm. Vanga selile na CCK-8 reagent (100 μ L / ml medium) kuri andi masaha 2, kandi kwinjiza byasomwe kuri 450 nm ukoresheje umusomyi wa microplate.Igipimo cyo gukumira ingirabuzimafatizo kibarwa ukurikije formula ikurikira: igipimo cyo kubuza ikwirakwizwa (%) = 1 - A2 nigiciro cya OD cyitsinda rishinzwe kugenzura ubusa, A3 nigiciro cya OD cya RPMI1640 giciriritse kitagira selile, naho A4 nigiciro cya OD cyibiyobyabwenge bifite intumbero imwe na A1 ariko idafite selile.Agaciro IC50 yabazwe nisesengura ridasubirwaho hifashishijwe porogaramu ya grappad prism [1], igereranya kwibanda ku biyobyabwenge byerekana 50% bibuza gukura kwa selile.

Reba:[1].Xie J, n'abandi.Ingaruka ya antitumor ya tanshinone IIA mukurwanya ikwirakwizwa no kugabanya imvugo ya VEGF / VEGFR2 kuri kanseri y'ibihaha ya muntu itari ntoya A549 umurongo w'akagari.Acta Pharm Icyaha B. 2015 Ugushyingo;5 (6): 554-63.
[2].Zhang Z, n'abandi.Tanshinone IIA ibuza apoptose muri myocardium itera microRNA-152-3p imvugo bityo ikagabanya PTEN.Am J Umusemuzi Res.2016 Nyakanga 15;8 (7): 3124-32.
[3].Su CC, n'abandi.Tanshinone IIA igabanya imvugo ya poroteyine ya EGFR, na IGFR ikabuza inzira ya PI3K / Akt / mTOR mu ngirabuzimafatizo ya kanseri yo mu nda AGS haba muri vitro ndetse no muri vivo.Oncol Rep. 2016 Kanama;36 (2): 1173-9.