Alaabta

Magaca Ingiriisiga:tanshinone IIA
Magaca Shiinaha:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA dhaqdhaqaaqa noolaha

Sharaxaad:
Tanshinone IIA (Tan IIA) waa mid ka mid ah halabuurka dufanka ugu weyn ee xididada xididka cas ee Salvia miltiorrhiza.Tanshinone IIA waxay joojin kartaa angiogenesis iyada oo la beegsanayo qaybta kinase borotiinka ee VEGF / VEGFR2.

Qaybaha khuseeya:
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Alaabta Dabiiciga ah >> Quinones

Bartilmaameedka:
VEGF/VEGFR2[1]

Barashada Vitro dhexdeeda:Saamaynta antitumor ee tanshinone IIA waxaa ka mid ah in la joojiyo kororka unugyada burooyinka, khalkhalka wareegga unugyada burooyinka, kor u qaadida apoptosis unugga buro iyo xakameynta duulaanka unugyada burooyinka iyo metastasis.Tanshinone IIa waxay saameyn liddi ku ah ku leedahay unugyada A549: IC50 ee tanshinone IIa ka dib 24, 48 iyo 72 saacadood waxay ahaayeen 145.3, 30.95 iyo 11.49, siday u kala horreeyaan μ M. CCK-8 ayaa loo isticmaalay si loo qiimeeyo tanshinone IIA Dhaqdhaqaaqa fidinta unugyada A549 ee la daweeyay 24, 48 iyo 72 saacadood, siday u kala horreeyaan.Natiijooyinka CCK-8 waxay muujiyeen in tanshinone IIA ay si weyn u joojin karto faafinta unugyada A549 ee qiyaasta ku-tiirsanaanta iyo wakhtiga ku-tiirsanaanta.Apoptosis muhiim ah iyo xakameynta koritaanka unugyada unugyada A549 ayaa la arkay 48 saacadood ka dib daaweynta daroogada (fiirsashada la isticmaalay waxay ku saabsan tahay qiimaha IC50: tanshinone iia31 on A549) μ M) μ M), hoos u habeeyaan muujinta VEGF iyo VEGFR2 borotiinka ee kooxda daawaynta daroogada iyo vector [1].Tanshinone IIA waa mid ka mid ah qaybaha ugu badan ee xididka Salvia miltiorrhiza, kaas oo ka ilaalin kara unugyada H9c2 apoptosis.Unugyada H9c2 ee lagu daaweeyay tanshinone IIA waxay joojiyeen angiotensin II apoptosis-ka keenay hoos u dhigista muujinta PTEN (phosphatase iyo tensin homologue).PTEN waa xakameyn buro oo door muhiim ah ka ciyaara apoptosis.Tanshinone IIA waxay joojisaa angiotensin II (AngII) - apoptosis-ka keentay hoos u dhigista muujinta phosphatase iyo tensin homologue (PTEN) [2].Tanshinone IIA waxay yaraynaysaa muujinta borotiinka ee EGFR, iyo IGFR waxay xannibaysaa dariiqa PI3K / Akt / mTOR ee kansarka caloosha ee unugyada AGS [3].

Tijaabada Unug:Unugyada A549 waxaa lagu tiriyey marxaladda logarithmic iyo 6000 unug (90 μL mugga) 96 saxan ceel ah.Doonaa 10 μL kala duwanaansho kala duwan oo tanshinone IIA ah (xeerka u dambeeya 80,60,40,30,20,15,10,5 iyo 2.5 μM) Iyo ADM (xeerka u dambeeya 8,4,2,1,0.5 iyo 0.25 μM ) Waxaa lagu daray kooxda dawada, halka kooxda xakamaynta taban (kooxda side) lagu daray kaliya 10 μ Ldmso ama cusbada caadiga ah oo aan lahayn tanshinone IIA ama Adm. Isku qas unugyada CCK-8 reagent (100 μ L / ml dhexdhexaad ah) 2 saacadood oo kale, iyo nuugista ayaa lagu akhriyay 450 nm iyadoo la adeegsanayo akhristaha microplate.Heerka xakameynta kororka unugyada waxaa loo xisaabiyaa iyadoo loo eegayo qaacidada soo socota: heerka xakameynta faafinta (%) = 1 - [(A1-A4) / (A2-A3)] × 100, halkaasoo A1 ay tahay qiimaha OD ee kooxda tijaabada ah ee daroogada, A2 waa qiimaha OD ee kooxda xakamaynta bannaan, A3 waa qiimaha OD ee RPMI1640 dhexdhexaad ah oo aan lahayn unugyo, iyo A4 waa qiimaha OD ee daawada oo la mid ah A1 laakiin aan lahayn unugyo.Qiimaha IC50 waxaa lagu xisaabiyay falanqaynta dib-u-celinta aan tooska ahayn iyadoo la adeegsanayo software-ka graphpad priism [1], taas oo ka dhigan xoojinta daroogada oo muujinaysa 50% joojinta koritaanka unugyada.

Tixraac:[1]Xie J, iyo al.Saamaynta antitumor ee tanshinone IIA ee ka-hortagga ka-hortagga iyo hoos u dhigista muujinta VEGF/VEGFR2 ee kansarka sambabada unugyada aan-yaraha ahayn ee bini'aadamka A549.Acta Pharm Sin B. 2015 Nof;5 (6): 554-63.
[2].Zhang Z, iyo al.Tanshinone IIA waxay joojisaa apoptosis ee myocardium iyada oo kicinaysa muujinta microRNA-152-3p oo hoos u dhigta PTEN.Am J Transl Res.2016 Jul 15;8 (7): 3124-32.
[3]Su CC, iyo al.Tanshinone IIA waxay hoos u dhigtaa muujinta borotiinka ee EGFR, iyo IGFR oo xannibaysa dariiqa PI3K/Akt/mTOR ee kansarka caloosha ee AGS unugyada labadaba gudaha vitro iyo vivo.Oncol Rep. 2016 Agoosto;36 (2): 1173-9.