Lihlahisoa

Tlhaloso:
pachymic acid ke triterpenoid e tsoang ho P. cocos.Pachymic acid e thibela litsela tsa lipontšo tsa Akt le ERK.

Lihlopha tse amanang:
lefapha la lipatlisiso > > kankere
Lihlahisoa tsa Tlhaho > > terpenoids le glycosides

Sepheo:
Akt
ERK

Thuto ea In Vitro:
pachymic acid (PA) e ka thibela tumorigenesis ea mofetše oa nyooko e kenyelletsang litsela tsa lipontšo tsa Akt le ERK.Kalafo ea Pachymic acid (PA) e ile ea thibela haholo litsela tsa Rho a, Akt le ERK liseleng tsa mofetše oa gallbladder.Kalafo ea Pachymic acid (PA) e ka fokotsa PCNA, ICAM-1, RhoA, p-Akt le perk ka mokhoa o itšetlehileng ka tekanyo.Lihora tse 12 ka mor'a phekolo, 10 μ G / ml Poria acid (PA) e ile ea thibela kholo ea lisele ka har'a mahloriso a 30 μ G / ml ho fokotsa ho hōla ha sele.Khōlo ea lisele e ile ea thibeloa ka nako - le mokhoa o itšetlehileng ka tekanyo.Ka mor'a lihora tse 48 tsa phekolo, mahloriso a ne a le 10 μ g / mL, 20 μ G / ml le 30 μ Pachymic acid (PA) ho g / ml e thibela kholo ea lisele ka hoo e ka bang 25%, 40% le 70%.Pachymic acid (PA) e boetse e thibela kholo ea lisele tsa mofetše oa gallbladder ka mokhoa o itšetlehileng ka nako le o itšetlehileng ka tekanyetso [1].

Thutong ea Vivo:
e le ho hlahloba tšebetso ea antitumor ea pachymic acid (PA) in vivo, ho ile ha sebelisoa mofetše oa matšoafo oa motho oa nci-h23 tumor xenograft.Pachymic acid (PA) e thibetse haholo kholo ea tumor ka litekanyetso tsa 30 le 60 mg / kg ka matsatsi a 21 ha e bapisoa le sehlopha sa taolo [2].

Teko ea Cell:
cell counting kit-8 (CCK-8) e ne e sebelisetsoa ho hlahloba phello ea anti proliferation ea pachymic acid (PA) liseleng tsa GBC-SD.Ka bokhutšoanyane, ka mor'a phekolo e boletsoeng, eketsa 10 ho lesoba le leng le le leng la tharollo ea μ Lcck-8, 'me ka mor'a ho incubation e le' ngoe, ho monya ho ne ho lekanyetsoa ho 450 nm ho sebelisa sebali sa microplate [1].

Teko ea Liphoofolo:
litoeba [2] li sebelisitse litoeba tsa basali tse hlobotseng tsa athymic tse lilemo li 4-5 libeke.Lisele tsa nci-h23 tse holileng ka mokhoa o hlakileng (ho 100 μ 5 ho LPBs × 106) li kenngoa ka tlas'a letlalo lehlakoreng le letona la toeba ka 'ngoe.Li-tumor xenografts li ne li hōlile ho ea ho boholo ba karolelano ea 100-200mm 3 'me ka linako tse ling li abeloa lihlopha tse' nè tse fapaneng tsa phekolo (litoeba tse 6 sehlopheng ka seng): (a) taolo ea koloi (0.1% DMSO ka saline e tloaelehileng);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg / kg;(Dd) PA 60mg / kg. Litoeba li ile tsa fuoa ente ea intraperitoneal (IP) bakeng sa libeke tsa 3 (matsatsi a 5 / beke).Boholo ba hlahala bo ne bo lekantsoe ka lilepe tse peli ka thuso ea vernier caliper, 'me molumo oa tumor (mm3) o ne o baloa.

Referense:
[1].Chen Y, le ba bang.Pachymic acid e thibela tumorigenesis liseleng tsa gallbladder carcinoma.Int J Clin Exp Med.2015 Oct 15;8(10):17781-8.
[2].Ma J, le ba bang.Pachymic acid e etsa hore ho be le apoptosis ka ho kenya tšebetsong litsela tsa khatello ea maikutlo tsa JNK le ER tse itšetlehileng ka ROS liseleng tsa mofetše oa matšoafo.Kankere Cell Int.2015 Aug 5;15:78.