iGlycyrrhizin, iLiquiritin;iLiquiritoside;iLikviritin;Liquiritoside Cas No.551-15-5
Intshayelelo emfutshane
IGlycyrrhizin, eyaziwa ngokuba yiLiquiritin.ILicorice sisityalo seGlycyrrhiza kwiLeguminosae.Iingcambu zayo kunye neziqu ziyimifuno eqhelekileyo yaseTshayina.
Amayeza asasazwa ngokubanzi kwi-Northeast China, i-Xinjiang, i-Yunnan, i-Inner Mongolia, i-Anhui nakwezinye iindawo.I-Shennong materia medica classic iyidwelisa njengelona bakala liphezulu, isithi “le ngca yinkosi yawo onke amayeza, kwaye bambalwa abangayisebenzisiyo”.I-Licorice inamacandelo anzima, ngokukodwa kuquka i-triterpenoids, i-flavonoids kunye ne-coumarins.I-Flavonoids luhlobo lwamacandelo e-bioactive afunyenwe kwi-licorice extract.Amacandelo ayo amachiza amayeza abandakanya i-glycyrrhizin, i-isoglycyrrhizin, i-glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, njl. Kwiminyaka yakutshanje, kukho iingxelo malunga nokuchithwa kwe-radical yamahhala, i-antioxidant, i-anti-cancer kunye ne-anti mutagenic ye-licorice flavonoids ekhaya nakwamanye amazwe.
IkhemikhaliName:4H-1-Benzopyran-4-enye, 2- [4-(β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPimpahla:Monohydrate (dilute ethanol okanye amanzi), indawo yokunyibilika: 212 ~ 213 ° ℃.
Isenzo se-Pharmacological
Ubutyhefu: Akukho
Ukusabela kakubi: ayaziwa
Umthombo wesithako: i-legume Glycyrrhiza glabra L. ingcambu, iGlycyrrhiza uralensis Fisch Root.
Ukutsalwa kweGlycyrrhizin
Ukunyangwa kweLicorice Raw Materials
Ukwakhiwa kweekhemikhali zezinto eziluhlaza ze-licorice zinzima kakhulu.Ukuze ufumane isiphumo esingcono sokwahlula, ukunciphisa ukungcola kokungcola kwikholamu yokulungiselela i-chromatographic, kunye nokuphucula umxholo we-glycyrrhizin kwi-injection ekrwada, indlela yokukhupha yayisetyenziselwa ukugqithiswa kwezinto eziluhlaza.Yenza i-4G hay kunye nebhalansi ye-elektroniki kwaye uyifake kwi-beaker.Yenza umlinganiselo ngokuchanekileyo we-100ml wamanzi adibeneyo kunye ne-cylinder yokulinganisa kwaye uwagalele kwi-beaker ukuze anyibilike.I-Ultrasonic malunga nemizuzu eyi-15, kwaye rhoqo uvuselela ngentonga yeglasi ukukhawulezisa ukuchithwa.Emva koko faka ibhekile kwibhafu ye-thermostatic eyi-90 ℃ kwaye uyifudumeze iiyure ezi-2, emva koko uyifudumeze ukuze ihluzwe.Emva kokongeza i-filtrate kwi-n-butanol solvent kunye nokuma imizuzu emininzi, ininzi ye-glycosides inyibilika kwi-n-butanol solvent, emva koko iqhube i-secondary extraction, ikhuphe inani elincinci le-glycosides eseleyo emanzini, kwaye ekugqibeleni idibanise kwaye igxininise i-n- isisombululo se-butanol esifunyenwe ngokutsalwa okwesibini kwechromatography kunye nokuhlanjululwa.
Ukucocwa kweGlycyrrhizin ngeChromatography
Thatha i-10 ml yemveliso ekhutshiweyo ngasentla njengesixhobo esingasetyenziswanga, qalisa impompo, seta isantya sokuhamba kwi-25 ml / min, kwaye uzise imathiriyeli ekrwada ibe yi-500 mm ngesigaba esihambayo (methanol: amanzi = 1: 4) × Ikholamu yokulungiselela i-40 mm, qokelela iqhezu lemveliso ye-hay glucoside ngokwemeko yencopho: iqhezu le-1 h yokuqala iqokelelwa kunye njengeqhezu lokungcola kwangaphambili, kwaye emva koko utshintshe ukuhamba.Umzekelo, hlamba ikholamu ngomxube we-50% ye-methanol kunye namanzi, udibanise imveliso rhoqo ngemizuzu engama-20, kwaye emva koko ugxininise ibhotile nganye yemveliso nge-evaporation ejikelezayo, kwaye uthathe i-20 µ L yohlalutyo lwe-chromatographic ye-HPLC, Kude kube akukho thagethi ifunyenweyo.Iimeko zokufumanisa i-HPLC zimi ngolu hlobo lulandelayo: isigaba seselula: i-methanol: amanzi = 3.5: 6.5;Isigaba sokumisa: i-silica gel carbon 18;Ikholamu yeChromatographic: 450 mm × 4.6 mm; Isantya sokuhamba: 1 ml / min;Ubungakanani bewavenge: 254nm.Umxholo we-glycyrrhizin kwibhotile yesibini yeyona iphakamileyo phakathi kweemveliso ezifunyenwe yonke i-20 min
Ukucocwa kweGlycyrrhizin ngeRechromatography
Ekubeni umxholo we-glycyrrhizin emva kokucoca i-chromatographic ephambili ayiphezulu, indlela efanayo ikhethiweyo.Thatha i-10 ml yemveliso ehlanjululweyo engentla njengezinto ezilindileyo, izinga lokuhamba li-25 ml / min, kwaye uzise ibhotile yesibini yemveliso kwi-500 mm ngesigaba esihambayo (i-methanol: amanzi = 2: 5) × Kwi-20 mm. } ikholamu yechromatographic, qokelela i-distillate yemveliso ye-hay glycoside ngokwemeko yencopho: qhagamshela imveliso rhoqo ngemizuzu emi-4, emva koko ugxininise ibhotile nganye yemveliso ngomphunga ojikelezayo, kwaye usebenzise umcu ofanayo wokubhaqa ngaphezulu kuhlalutyo lwe-HPLC de kungabikho thagethi. .Emva kokuhlalutya, kwafunyaniswa ukuba umxholo we-glycyrrhizin kwibhotile yesithandathu wawuphezulu phakathi kweemveliso ezifunyenwe yonke i-4 min, apho ixesha lokugcinwa laliyi-5.898 min njengeyona nto iphakamileyo ekujoliswe kuyo, kwaye umxholo ufikelele malunga ne-40% ngendlela yokulinganisa indawo. .
Unyango lwePosi yeeMveliso
Imveliso eqokelelweyo idistilled phantsi koxinzelelo oluncitshisiweyo kwi-evaporator ejikelezayo kwi-70 ℃.Emva kokuba i-solvent iguqulwe ibe ngumphunga, nyibilikisa imveliso eqinileyo kwiflask esezantsi engqukuva ngexabiso elincinci le-methanol, kwaye ukhazimle kwityhubhu yovavanyo kwiqondo lobushushu begumbi kude kuvele iikristale ezimhlophe zegranular [2].
