Imikhiqizo

Incazelo:
i-pachymic acid iyi-triterpenoid evela ku-P. cocos.I-Pachymic acid ivimbela izindlela zokusayina ze-Akt ne-ERK.

Izigaba Ezihlobene:
umkhakha wocwaningo > > umdlavuza
Imikhiqizo yemvelo > > ama-terpenoids nama-glycosides

Ithagethi:
Akt
ERK

Isifundo se-In Vitro:
i-pachymic acid (PA) ingavimbela i-tumorigenesis yomdlavuza wenyongo ehlanganisa izindlela zokusayina ze-Akt ne-ERK.Ukwelashwa kwe-Pachymic acid (PA) kuvimbele kakhulu izindlela ze-Rho a, Akt kanye ne-ERK kumaseli omdlavuza we-gallbladder.Ukwelashwa kwe-Pachymic acid (PA) kungehlisa i-PCNA, i-ICAM-1, i-RhoA, i-p-Akt kanye ne-perk ngendlela encike kumthamo.Emahoreni ayi-12 ngemuva kokwelashwa, i-10 μ G / ml i-Poria acid (PA) ivimbele ukukhula kwamaseli ekugxilweni kuka-30 μ G / ml futhi kunciphise ukukhula kwamaseli.Ukukhula kweseli kuye kwavinjwa ngesikhathi - nangendlela encike kumthamo.Ngemuva kwamahora angama-48 wokwelashwa, ukugxila kwaba ngu-10 μ g/mL, 20 μ G/ml kanye no-30 μ Pachymic acid (PA) ku-g/ml evimbele ukukhula kwamaseli cishe ngo-25%, 40% no-70%.I-Pachymic acid (PA) iphinde ivimbele ukukhula kwamaseli omdlavuza we-gallbladder ngendlela encike esikhathini futhi encike kumthamo [1].

Esifundweni Se-Vivo:
ukuze kuhlolwe umsebenzi we-antitumor we-pachymic acid (PA) ku-vivo, imodeli yomdlavuza wamaphaphu womuntu i-nci-h23 tumor xenograft yasetshenziswa.I-Pachymic acid (PA) ivimbele kakhulu ukukhula kwesimila kumithamo engama-30 no-60 mg / kg izinsuku ezingama-21 uma kuqhathaniswa neqembu lokulawula [2].

Ukuhlolwa Kweseli:
Ikhithi yokubala yamaseli-8 (CCK-8) isetshenziselwe ukuhlola umphumela wokulwa nokwanda kwe-pachymic acid (PA) kumaseli e-GBC-SD.Ngamafuphi, ngemva kokwelashwa okucacisiwe, engeza i-10 emgodini ngamunye μ Lcck-8 isisombululo, futhi ngemva kokufakwa okukodwa, ukumuncwa kukalwa ku-450 nm kusetshenziswa umfundi we-microplate [1].

Ukuhlolwa Kwezilwane:
amagundane [2] asebenzise amagundane anqunu wesifazane aneminyaka engama-4-5.Amaseli e-nci-h23 akhule ngendlela emangalisayo (ku-100 μ 5 kuma-LPBs × 106) ajovwe ngaphansi kwesikhumba ohlangothini olungakwesokudla lwegundane ngalinye.I-Tumor xenografts yakhuliswa yaba usayizi ojwayelekile we-100-200mm 3 futhi yanikezwa ngezikhathi ezithile kumaqembu amane okwelapha ahlukene (amagundane angu-6 eqenjini ngalinye): (a) ukulawula imoto (0.1% DMSO ku-saline evamile);(b) I-Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg/kg;(Dd) PA 60mg / kg. Amagundane anikezwe umjovo we-intraperitoneal (IP) amaviki angu-3 (izinsuku ezingu-5 / isonto).Ubukhulu besimila bulinganiswa ngezimbazo ezimbili ngosizo lwe-vernier caliper, futhi ivolumu yesimila (mm3) ibaliwe.

Ireferensi:
[1].U-Chen Y, et al.I-Pachymic acid ivimbela i-tumorigenesis kumaseli e-gallbladder carcinoma.I-Int J Clin Exp Med.2015 Okthoba 15;8(10):17781-8.
[2].UMa J, et al.I-Pachymic acid yenza i-apoptosis ngokuvula izindlela zokucindezeleka ezincike ku-ROS kanye ne-ER kumaseli omdlavuza wamaphaphu.ICancer Cell Int.2015 Aug 5;15:78.