Imikhiqizo

Igama lesiNgisi:i-tanshinone IIA
Isiteketiso sesiShayina:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA umsebenzi webhayoloji

Incazelo:
I-Tanshinone IIA (i-Tan IIA) ingenye yezingoma eziphambili ezincibilikayo ezimpandeni zempande ebomvu i-Salvia miltiorrhiza.I-Tanshinone IIA ingavimbela i-angiogenesis ngokukhomba isizinda seprotein kinase se-VEGF/VEGFR2.

Izigaba ezifanele:
Umkhakha wocwaningo > > isifo senhliziyo
Imikhiqizo Yemvelo > > Ama-Quinones

Ithagethi:
I-VEGF/VEGFR2[1]

Isifundo se-In Vitro:Imiphumela ye-antitumor ye-tanshinone IIA ihlanganisa ukuvimbela ukwanda kwe-tumor cell, ukuphazamisa umjikelezo wamaseli we-tumor, ukukhuthaza i-tumor cell apoptosis kanye nokuvimbela ukuhlasela kwe-tumor cell kanye ne-metastasis.I-Tanshinone IIA ibe nomphumela wokulwa nokwanda kwamaseli e-A549: i-IC50 ye-tanshinone IIA ngemva kwamahora angu-24, 48 kanye namahora angu-72 yayingu-145.3, 30.95 kanye no-11.49, ngokulandelana μ M. CCK-8 assay yasetshenziswa ukuhlola i-tanshinone IA80 μ-M-M80 (25). Umsebenzi okhulayo wamaseli e-A549 aphathwe ngamahora angama-24, 48 kanye nangama-72, ngokulandelana.Imiphumela ye-CCK-8 ibonise ukuthi i-tanshinone IIA ingavimbela ngokuphawulekayo ukwanda kwamaseli e-A549 ngendlela encike kumthamo nangendlela encike esikhathini.I-apoptosis ebalulekile kanye nokuvimbela ukukhula kwamaseli amaseli e-A549 kwabonwa emahoreni angu-48 ngemva kokwelashwa kwezidakamizwa (ukugxilisa ingqondo okusetshenzisiwe kwakucishe kube nenani le-IC50: tanshinone iia31 ku-A549) μ M) . μ M) , phansi lawula ukuvezwa kwe-VEGF ne-VEGFR2 amaprotheni eqenjini lokwelashwa kwezidakamizwa kanye ne-vector [1].I-Tanshinone IIA ingenye yezingxenye ezitholakala ngobuningi bezimpande ze-Salvia miltiorrhiza, ezingavikela amaseli e-H9c2 ku-apoptosis.Amaseli e-H9c2 alashwe nge-tanshinone IIA avimbele i-angiotensin II enza i-apoptosis ngokwehlisa ukuqondisa kwe-PTEN (i-phosphatase ne-tensin homologue).I-PTEN isicindezeli sesimila esidlala indima ebalulekile ku-apoptosis.I-Tanshinone IIA ivimbela i-angiotensin II (AngII) - i-apoptosis eyenziwe ngokunciphisa i-phosphatase ne-tensin homologue (PTEN) [2].I-Tanshinone IIA yehlisa ukubonakaliswa kwamaprotheni e-EGFR, futhi i-IGFR ivimba indlela ye-PI3K / Akt / mTOR kumaseli womdlavuza wesisu we-AGS [3].

Ukuhlolwa Kweseli:Amaseli e-A549 abalwe esigabeni se-logarithmic namaseli angu-6000 (ivolumu engu-90 μ L) epuleti lomthombo elingu-96.Ingabe 10 μ L ukugxila okuhlukile kwe-tanshinone IIA (ukugxila kokugcina 80,60,40,30,20,15,10,5 kanye no-2.5 ​​μ M) Kanye ne-ADM (ukugxila kokugcina 8,4,2,1,0.5 kanye no-0.25 μ M ) Yengezwe eqenjini lezidakamizwa, kuyilapho iqembu lokulawula elingalungile (iqembu lenkampani yenethiwekhi) lanezelwa kuphela i-10 μ Ldmso noma i-saline evamile ngaphandle kwe-tanshinone IIA noma i-Adm. Hlanganisa amaseli ne-CCK-8 reagent (100 μ L / ml medium) ukuze amanye amahora we-2, futhi ukumuncwa kwafundwa ku-450 nm kusetshenziswa umfundi we-microplate.Izinga lokuvimbela ukwanda kwamaseli libalwa ngokuvumelana nefomula elandelayo: izinga lokuvimbela ukwanda (%) = 1 - [(A1-A4) / (A2-A3)] × 100, lapho i-A1 iyinani le-OD leqembu lokuhlola izidakamizwa, I-A2 inani le-OD leqembu lokulawula elingenalutho, i-A3 iyinani le-OD le-RPMI1640 medium ngaphandle kwamaseli, futhi i-A4 iyinani le-OD lomuthi elinokugxila okufanayo njenge-A1 kodwa ngaphandle kwamaseli.Inani le-IC50 libalwe ngokuhlaziywa kokuhlehla okungekona umugqa kusetshenziswa isofthiwe ye-graphpad prism [1], emele ukugxiliswa kwezidakamizwa okubonisa ukuvimbela ukukhula kwamaseli okungama-50%.

Ireferensi:[1].Xie J, et al.Umthelela we-antitumor we-tanshinone IIA ekulweni nokwanda kanye nokuncipha kwenkulumo ye-VEGF/VEGFR2 kumugqa weseli womuntu ongewona omncane wamaphaphu we-A549.Acta Pharm Sin B. 2015 Nov;5(6):554-63.
[2].UZhang Z, et al.I-Tanshinone IIA ivimbela i-apoptosis ku-myocardium ngokufaka inkulumo ye-microRNA-152-3p futhi ngaleyo ndlela yehlise i-PTEN.Am J Transl Res.2016 Jul 15;8(7):3124-32.
[3].Su CC, et al.I-Tanshinone IIA yehlisa ukubonakaliswa kwamaprotheni e-EGFR, kanye ne-IGFR ivimba indlela ye-PI3K/Akt/mTOR kumaseli e-gastric carcinoma AGS kokubili ku-vitro kanye ne-vivo.I-Oncol Rep. 2016 Aug;36(2):1173-9.