Imikhiqizo

I-Typhonine glycoside

I-Puhuang impova eyomisiwe ye-Typha angustifolia L., i-Typha Orientalis Presl noma izitshalo ezifanayo ku-Typhaceae.Okuqukethwe kwe-isorhamnetin-3-o-neohesperidin in Chinese Pharmacopoeia (2005 Edition) kunqunywa i-HPLC.Izindlela eziyinhloko zokunqunywa kwe-isorhamnetin-3-o-neohesperidin ku-Typhae kanye namalungiselelo ayo

I-HPLC.Ngaphansi kokunqunywa kokuqukethwe kwe-Typha in Chinese Pharmacopoeia (Ushicilelo luka-2005):

Izimo ze-Chromatographic nokuhlolwa kokusebenza kwesistimu: i-octadecyl silane eboshiwe ijeli ye-silica njengesigcwalisi;Amanzi e-acetonitrile (15:85) asetshenziswe njengesigaba esihambayo;Ubude bokutholwa bebungu-254 nm.Izinga lokushisa lekholomu lingu-40 ℃.Inombolo yamapuleti etiyori akufanele ibe ngaphansi kuka-1000 ngokuya ngenani eliphakeme le-isorhamnetin-3-o-neohesperidin.

Ukulungiswa kwesixazululo sokuhlola: thatha cishe u-0.5g walo mkhiqizo, ukale kahle, uwubeke ebhodleleni lokulinganisa elingu-50ml, engeza u-45ml we-methanol, ukwelashwa kwe-ultrasonic (amandla 250W, imvamisa 20KHz) imizuzu engama-30, uyipholise, engeza i-methanol sika, yinyakazise kahle, ihlunge, futhi uthathe isihlungi esiqhubekayo

Ukunqunywa kwe-isorhamnetin-3-o-neohesperidin e-Puhuang kanye namalungiselelo ayo yi-HPLC.Uhlelo olujwayelekile lwesigaba seselula amanzi e-acetonitrile:

① Hp1050 ukusebenza okuphezulu kwechromatograph ewuketshezi;Ikholomu yeChromatographic C18 (5) μ m,4.6mm × 250mm) I-Dalian Institute of Chemical Physics, i-Chinese Academy of Sciences);Isigaba seselula: amanzi e-acetonitrile (15:85);Ubude begagasi bokubona: 254nm;Ikholomu lokushisa lokushisa.

② I-chromatograph ewuketshezi esebenza kakhulu isistimu ye-hp1050 ye-Hewlett Packard Company e-United States;Ikholomu ye-Chromatographic bondclone 10 C18 (3.9mm) × 300mm）; Isigaba seselula: i-acetonitrile yamanzi (80:20);ukutholwa kwe-wavelength 254 nm, umkhawulokudonsa ongu-4 nm;ireferensi wavelength 550 nm, bandwidth 100 nm;ukwehlisa 8.

Fu Daxu et al.Kunqunywe okuqukethwe kwe-typhoniflorin ne-isorhamnetin-3-o-neohesperidin kungqikithi ye-flavone ekhishwe yi-Typha nge-HPLC kanye ne-1100 chromatograph.Ikholomu ye-chromatographic yi-eclipse XdB C18 (250mm × 4.6mm,5 μ m); Isigaba seselula: i-acetonitrile-0.5% i-acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Ubude begagasi bokutholwa bungu-248 nm.Ukulungiswa kwengqikithi ye-flavonoids ekhishwe ye-Typha: i-50g ye-Typha ikhishwe nge-ethanol engu-60% (500ml) × 2), i-1H ​​isikhathi ngasinye, ukukhishwa kwakugxilwe ku-40 ℃, ukukhishwa kwachithwa ngamanzi angu-600ml kanye ne-centrifuged.Izinsalela zachithwa ngamanzi angu-300ml kanye ne-centrifuged.Hlanganisa isixazululo se-centrifugal, lungisa ivolumu ibe ngu-1000ml ngamanzi, uyikhangise kukholamu ye-resin macroporous (150ml macroporous resin dryweight 75g) nenani eliphindwe izikhathi ezingu-1.5 kunenani lezidakamizwa ezingcolile, i-elute ngamanzi angu-600ml kanye no-450ml we-ethanol engu-20% ukungcola, bese elute nge 600ml ka 50% ethanol, futhi eluent itholakala vacuum drying at 40 ℃.

Yang Yonghua et al.Kusetshenziswe ukusebenza okuphezulu kwe-capillary electrophoresis nokusebenza okuphezulu kwe-chromatography ye-liquid ukuze kunqunywe okuqukethwe kwe-isorhamnetin 232o2, neohesperidin ne-typhoside e-Puhuang.I-Hpce-3d high performance capillary electrophoresis instrument (Hewlett Packard, USA), ikholomu ye-quartz capillary 50 μ m × 56cm (HP), lc-6a ukusebenza okuphezulu kwe-chromatograph ewuketshezi (inkampani yase-Shimadzu yase-Japan), i-C18 ikholomu 5mm × 200mm, usayizi wezinhlayiyana 5 μ M (inkampani yakwaDalian Yite).Izimo ze-HPCE: i-buffer 0.02mol/l borax-0.05mol/l 10% i-acetonitrile ye-sodium dodecyl sulfate (SDS), i-voltage 20kV, izinga lokushisa lekholomu 30 ℃, ubude bokutholwa okungu-270nm, umthamo womjovo 30KPa · s.Izimo ze-HPLC: isigaba seselula kwakungamanzi e-acetonitrile (15:85), izinga lokushisa lekholomu lalingu-25 ℃, futhi ubude begagasi bokutholwa bungu-254 nm.

I-Typhaneoside Reference substance

[igama]i-typhonioside [1]

[Inombolo ye-CAS.]104472-68-6

[indlela yokuthola]I-HPLC ≥ 98%

[Ukucaciswa]20mg 50mg 100mg 500mg 1g (ingahlanganiswa ngokuvumelana nezidingo zekhasimende)

[izakhiwo]i-amorphous powder