Verbascoside CAS No. 61276-17-3
Ulwazi Olubalulekile
[igama]I-Mullein glycoside
[isibizo]i-ergosterol, i-mullein
[isigaba]phenylpropanoid glycosides
[Igama lesiNgisi]i-acteoside;I-Verbascoside;Kusaginin
[ifomula yamangqamuzana]I-C29H36O15
[isisindo samangqamuzana]624.59
[Inombolo ye-CAS.]61276-17-3
Izakhiwo ze-Physicochemical
[izakhiwo]lo mkhiqizo uyimpushana ye-crystal enaliti emhlophe
[ukuminyana okuhlobene]1.6g/cm3
[ukuncibilika]Incibilika kalula ku-ethanol, i-methanol ne-ethyl acetate.
Umthombo Wokukhipha
Lo mkhiqizo uyisiqu esomile esiyinyama esinamaqabunga anamaxolo e-Cistanche deserticola, isitshalo somndeni wakwa-liedang.
Indlela Yokuhlola
I-HPLC ≥ 98%
Izimo ze-Chromatographic: isigaba seselula i-methanol acetonitrile 1% i-acetic acid (15:10:75), izinga lokugeleza ngu-0.6 ml · min-1, izinga lokushisa lekholomu 30 ℃, ubude begagasi bokutholwa ngu-334 nm (ngokwereferensi kuphela)
Umsebenzi kanye nokusetshenziswa
Lo mkhiqizo usetshenziselwa ukunquma okuqukethwe
Indlela Yesitoreji
2-8 ° C, agcinwe kude nokukhanya.
I-Bioactivity ye-Verbascoside
Isifundo se-In Vitro:
Njenge-PKC Inhibitor ye-ATP eqhudelanayo, i-Verbascoside ine-IC50 ka-25 μ M [1]。 I-Verbascoside (5,10) μ M) Ukuvinjelwa kwe-2,4-dinitrochlorobenzene (DNCB) - i-T cell costimulatory factor eyenziwe nge-CD86 ne-CD54, ama-cytokines ane-proinflammatory kanye ne-NF kumaseli we-thk-1 κ B indlela yokusebenza [2].
Ezifundweni ze-Vivo:
I-Verbascoside (1%) yehlise izehlakalo zokuziphatha kokuklwebheka kanye nokuqina kwezilonda esikhumbeni kumodeli yegundane le-2,4-dinitrochlorobenzene (DNCB) - i-atopic dermatitis (AD).I-Verbascoside ingaphinda ivimbele i-cytokine TNF e-pro-inflammatory ku-DNCB izilonda zesikhumba ezibangelwa- α, Ukuvezwa kwe-IL-6 kanye ne-IL-4 mRNA [2].I-Verbascoside (50100 mg / kg, IP) ayizange iguqule ubuhlungu obubandayo obungavamile obubangelwa ukulimala okungapheli kokucindezela (CCI).I-Verbascoside(200 mg/kg, IP) yehlise ukungezwani komzimba ne-acetone evuselelwe amakhaza ngosuku lwesi-3. I-Verbascoside iphinde yanciphisa kakhulu izinguquko zokuziphatha ezihambisana ne-neuropathy.Ngaphezu kwalokho, i-Verbascoside yehlise i-Bax futhi yanda i-Bcl-2 ngosuku lwe-3 [3].
Ukuhlolwa Kweseli:
i-lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) iqukethe u-10% we-fetus bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ Eqoqweni elingu-24 lomthombo we-Dulbecco eliguquliwe Ukhozi, amaseli angu-104 emthonjeni ngamunye ayembalwa. I-Ml streptomycin sulfate ne-Verbascoside (encibilike ku-DMSO).Ukukhula kwaqashelwa ngokubala inani lamaseli kukhawunta ye-Coulter ngemva kwezinsuku ezi-2 zokufukamela endaweni eswakeme (5% CO2 emoyeni) ku-37 ℃.Inani le-IC50 libalwe ngokusekelwe emugqeni wokuhlehla womugqa osungulwe kunhlanganisela ngayinye yokuhlola [1].
Ukuhlolwa Kwezilwane:
Ukuze kudaleke i-atopic dermatitis (AD) - njengezimpawu, amagundane [2] asebenzisa i-2,4-dinitrochlorobenzene (DNCB).Ngamafuphi, izinwele ze-dorsal zamagundane zasuswa ngesikelo se-electronic izinsuku ezingu-2 ngaphambi kokwelashwa kwe-DNCB.I-Will 200 μ L ka-1% DNCB (ku-acetone: amafutha omnqumo = 4:1) yafakwa esikhumbeni sangemuva esiphuciwe ukuze siqwashise.Ukuhlaselwa okuphindaphindiwe kwenziwa endaweni efanayo, i-0.2% DNCB njalo ezinsukwini ezingu-3 cishe amasonto angu-2.Amagundane ahlukaniswe ngamaqembu angu-4 (n = 6 eqenjini ngalinye): (1) ukulawula imoto ephathwayo, (2) i-DNCB iphathwa kuphela, (3) 1% i-Verbascoside (i-acetone: amafutha omnqumo 4: 1) - iphathwa kuphela, futhi ( 4) I-DNCB + 1% Iqembu eliphathwa nge-Verbascoside[2].
Ireferensi:
[1].U-Herbert JM, et al.I-Verbascoside ihlukaniswe ne-Lantana camara, i-inhibitor ye-protein kinase C. J Nat Prod.1991 Nov-Dec;54(6):1595-600.
[2].Li Y, et al.I-Verbascoside Yehlisa Izimpawu Ezifana Ne-Atopic Dermatitis Kumagundane Ngomphumela Wawo Onamandla Wokulwa Nokuvuvukala.I-Int Arch Allergy Immunol.2018;175(4):220-230.
[3].U-Amin B, et al.Umthelela we-Verbascoside ku-Neuropathic Pain Obangelwa Ukulimala Okungapheli Kokuvinjelwa Kumagundane.I-Phytother Res.2016 Jan;30(1):128-35.
