page_head_bg

Products

Popular Design for Cas No.6750-30-7 - Tanshinone I – Yongjian

Short Description:


Product Detail

Product Tags

Related Video

Feedback (2)

Usually customer-oriented, and it's our ultimate focus on to be not only by far the most reliable, trustable and honest provider, but also the partner for our customers for Cas No.23313-21-5 , Harpagoside , Cas No.29708-87-0,123483-19-2 , We welcome you to definitely join us in this path of making a affluent and productive business together.
Popular Design for Cas No.6750-30-7 - Tanshinone I – Yongjian Detail:

Application of tanshinone I

Tanshinone I is a type IIA human recombinant sPLA2 and Rabbit Recombinant cPLA2 inhibitor with IC50 of 11, respectively μ M and 82 μ M.

Name of Tanshinone I

English Name: tanshinone I

Chinese Alias: tanshinone I | tanshinone I | 1,6-dimethyl-phenanthro [1,2-b] furan-10,11-dione | tanshinone I | tanshinone I | tanshinone I

Bioactivity Of Tanshinone I

Description: tanshinone I is a type IIA human recombinant sPLA2 and Rabbit Recombinant cPLA2 inhibitor with IC50 of 11 respectively μ M and 82 μ M。

Related Categories: signaling pathways > > metabolic enzymes /
proteases > > phospholipids
Research field > > cardiovascular disease
Natural Products > > Quinones

Target:IC50: 11 μM (sPLA2), 82 μM (cPLA2)[1].

In Vitro Study: tanshinone I inhibited the formation of PGE2 by LPS induced raw macrophages (IC50 = 38 μ M)。 When tanshinone I and LPS were added at the same time, the compound significantly inhibited 10-100 μ PGE2 production of M (IC50 = 38 μ M)。 When added after complete induction of COX-2, tanshinone I also reduced the production of PGE2 (IC50 = 46) μ M)。 The fact that tanshinone I inhibits PGE 2 production through pre induced COX-2 strongly suggests that the compound can directly inhibit COX-2 activity and / or affect PLA2 Activity. When tanshinone I was incubated with two different forms of phospholipase A2 (PLA2), it significantly inhibited sPLA2 in a concentration dependent manner (IC50 = 11) μ M). Despite its low potency, tanshinone I also inhibited cPLA2 (IC50 = 82) μ M)[1]

In Vivo Study: tanshinone I showed anti-inflammatory activity in carrageenan induced paw edema and adjuvant induced arthritis in rats. In order to establish the anti-inflammatory activity of tanshinone I, classical animal models of acute and chronic inflammation [rat carrageenan (CGN) - induced paw edema and rat adjuvant induced arthritis (AIA)] were used. When oral tanshinone I, it showed significant anti-inflammatory activity against CGN induced paw edema (47% inhibition at 160 mg / kg), while the IC50 of indomethacin was 7.1 mg / kg. In AIA, tanshinone I gave 27% inhibition of secondary inflammation on day 18 at an oral dose of 50 mg / kg / day, while prednisolone (5 mg / kg / day) showed effective inhibition (65%) [1]

Kinase Experiment: as the source of PLA2, human recombinant sPLA2 (type IIA) was purified from CHO cells transfected with PLA2 gene, and rabbit recombinant platelet cPLA2 was obtained through its expression in baculovirus. Standard reaction mixture (200) μ 50) It contained 100 mm Tris HCl buffer (pH 9.0), 6 mM CaCl2 and 20 nmol of 1-acyl – [1-14C] – arachidonyl Sn glycerol phosphate ethanolamine (2000 CPM / nmol). Or there was no tanshinone I. the reaction was started by adding 50NG purified sPLA2 or cPLA2. After 20 minutes at 37 ℃, the free fatty acids produced were analyzed. Under these standard conditions, about 10% of free fatty acids are released from the added phospholipid substrate in the reaction mixture without tanshinone I [1].

Cell Experiment: raw 264.7 cells were cultured with DMEM supplemented with 10% FBS and 1% antibiotics at 37 ℃ under 5% CO2. In short, cells were seeded on 96 well plates (2) × 10 (5 cells / well). Unless otherwise indicated, LPS (1ug / ml) and tanshinone I were added and incubated for 24 hours. The PGE2 concentration in the medium was measured using the EIA kit for PGE2. In order to determine the effect of tanshinone I on PGE 2 production after COX-2 induction, cells were mixed with LPS (1 μ G / ml) for 24 hours and washed thoroughly. Then, tanshinone I was added without LPS and the cells were cultured for another 24 hours. PGE2 concentration was measured from the medium. MTT assay was used to examine the cytotoxicity of tanshinone I on raw cells. Tanshinone I at 100 μ M did not show any cytotoxicity [1].

Animal Experiment: in order to evaluate the inhibitory activity of tanshinone I on acute and chronic inflammatory animal models, rat carrageenan (CGN) – induced paw edema and adjuvant induced arthritis (AIA) models were used. In short, 1% CGN dissolved in pyrogen free saline (0.05 ml) was injected into the right rear paw of rats for paw edema test. After 5 hours, the swelling of the treated claws was measured using a plethysmograph. Tanshinone I dissolved in 0.5% CMC was administered orally 1 hour before CGN injection. For the AIA test, arthritic inflammation was induced by injecting Mycobacterium lactis (0.6ml / rat) dissolved in mineral oil into the right hind paw of rats. Tanshinone I was administered orally every day. The expansion of treated and untreated claws was measured using a plethysmograph.

References: [1]  Kim SY, et al. Effects of Tanshinone I isolated from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses. Phytother Res. 2002 Nov; 16(7):616-20.

Physical And Chemical Properties Of Tanshinone I

Density: 1.3 ± 0.1 g / cm3

Boiling point: 498.0 ± 24.0 ° C at 760 mmHg

Melting point: 233-234 º C

Molecular formula: c18h12o3

Molecular weight: 276.286

Flash point: 245.9 ± 15.6 ° C

Exact mass: 276.078644

PSA:47.28000

LogP:4.44

Steam pressure: 0.0 ± 1.3 mmHg at 25 ° C

Refractive index: 1.676

Storage conditions: 2-8 ° C

Tanshinone I safety information

Hazard statement: h413

Transport code of dangerous goods: nonh for all modes of transport

Literature

Modulation of esterified drug metabolism by tanshinones from Salvia miltiorrhiza (“Danshen”).
J. Nat. Prod. 76(1) , 36-44, (2013)
The roots of Salvia miltiorrhiza (“Danshen”) are used in traditional Chinese medicine for the treatment of numerous ailments including cardiovascular disease, hypertension, and ischemic stroke. Extrac…

Tanshinone IIA inhibits viral oncogene expression leading to apoptosis and inhibition of cervical cancer.
Cancer Lett. 356(2 Pt B) , 536-46, (2015)
Human papilloma virus (HPV) is the well-established etiological factor of cervical cancer. E6 and E7 oncoproteins expressed by HPV are known to inactivate tumor suppressor proteins p53 and pRb, respec…

Cloning, molecular characterization and functional analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza Bge. f. alba.
Plant Physiol. Biochem. 70 , 21-32, (2013)
The enzyme 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) is a terminal-acting enzyme in the plastid MEP pathway, which produce isoprenoid precursors. The full-length cDNA of HDR, desi…

Cycloastragalol Literature

Cycloastragenol is a potent telomerase activator in neuronal cells: implications for depression management.

Neurosignals 22(1) , 52-63, (2014)
Cycloastragenol (CAG) is an aglycone of astragaloside IV. It was first identified when screening Astragalus membranaceus extracts for active ingredients with antiaging properties. The present study de…
A novel telomerase activator suppresses lung damage in a murine model of idiopathic pulmonary fibrosis.

PLoS ONE 8(3) , e58423, (2013)
The emergence of diseases associated with telomere dysfunction, including AIDS, aplastic anemia and pulmonary fibrosis, has bolstered interest in telomerase activators. We report identification of a n…
Telomerase-based pharmacologic enhancement of antiviral function of human CD8+ T lymphocytes.

J. Immunol. 181(10) , 7400-6, (2008)
Telomerase reverse transcribes telomere DNA onto the ends of linear chromosomes and retards cellular aging. In contrast to most normal somatic cells, which show little or no telomerase activity, immun…

 English Alias Of Tanshinone I

Salvia quinone

Phenanthro[1,2-b]furan-10,11-dione, 1,6-dimethyl-

Tanshinone

Tanshinon I

tanshinone-I

Tanshinone 1

1,6-Dimethylphenanthro[1,2-b]furan-10,11-dione

TANSHINONES IIA

Tanshinquinone I

MFCD00238692


Product detail pictures:

Popular Design for Cas No.6750-30-7 - Tanshinone I –  Yongjian detail pictures


Related Product Guide:

We have our own product sales staff, style crew, technical group, QC staff and package staff. We now have strict high quality management procedures for each approach. Also, all of our workers are experienced in printing subject for Popular Design for Cas No.6750-30-7 - Tanshinone I – Yongjian , The product will supply to all over the world, such as: Las Vegas, California, Jordan, Our company has built stable business relationships with many well-known domestic companies as well as oversea customers. With the goal of providing high quality products to customers at low cots, we've been committed to improving its capacities in research, development, manufacturing and management. We have honored to receive recognition from our customers. Till now we have now passed ISO9001 in 2005 and ISO/TS16949 in 2008. Enterprises of "quality of survival, the credibility of development" for the purpose, sincerely welcome domestic and foreign businessmen to visit to discuss cooperation.
  • This company has a lot of ready-made options to choose and also could custom new program according to our demand, which is very nice to meet our needs.
    5 Stars By Mike from Uzbekistan - 2018.05.13 17:00
    Factory equipment is advanced in the industry and the product is fine workmanship, moreover the price is very cheap, value for money!
    5 Stars By Kama from Zurich - 2018.07.26 16:51
    Write your message here and send it to us