Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Salvianolic acid B is the condensation of three molecules of Danshensu and one molecule of caffeic acid. It is one of the more studied salvianolic acids. It has important pharmacological effects on heart, brain, liver, kidney and other organs. This product has the effects of promoting blood circulation and removing blood stasis, dredging meridians and activating collaterals. It is mainly used to treat ischemic stroke caused by blood stasis blocking meridians, such as numbness of half body and limbs, weakness, contracture pain, motor failure, mouth and eye deflection, etc.
Alias: salvianolic acid B, salvianolic acid B, salvianolic acid B
English Name: salvianolic acid B
Molecular formula: c36h30o16
Molecular weight: 718.62
CAS No.: 115939-25-8
Detection method: HPLC ≥ 98%
Specifications: 10mg, 20mg, 100mg, 500mg, 1g (can be packaged according to customer requirements)
Function and use: This product is used for content determination.
Physical and chemical properties
Properties: the product is quasi white powder.
The taste is slightly bitter and astringent, with moisture inducing property. Soluble in water, ethanol and methanol.
Salvianolic acid B is formed by condensation of 3 molecules of salvianolic acid and 1 molecule of caffeic acid. It has two carboxyl groups and exists in the form of different salts (K +, Ca2 +, Na +, NH4 +, etc.). In the process of Decoction and concentration, a small part of salvianolic acid B is hydrolyzed to purple oxalic acid and salvianolic acid, and a part of salvianolic acid B becomes rosmarinic acid under acidic conditions; Salvianolic acid A and C can be tautomeric in solution.
The Radix Salviae Miltiorrhizae was crushed, put into the extraction tank, soaked with 8 times the amount of 0.01mol/l hydrochloric acid overnight, and then percolated with 14 times the amount of water. The percolated extracted solution is purified by AB-8 macroporous resin column. Firstly, elute with 0.01mol/l hydrochloric acid to remove the non adsorbed impurities, and then elute with 25% ethanol to remove the highly polar impurities. Finally, concentrate the 40% ethanol eluent under reduced pressure to recover ethanol and freeze-dry to obtain the total Salvia miltiorrhiza phenolic acid with a purity of more than 80%.
Take 1g of the product, grind it, add 5ml of ethanol, fully stir, filter, take a few drops of filtrate, dot it on the filter paper strip, dry it, observe it under the ultraviolet lamp (365nm), show blue-gray fluorescence, hang the filter paper in the concentrated ammonia solution bottle (not contacting the liquid surface), take it out after 20 minutes, observe it under the ultraviolet lamp (365nm), show blue-green fluorescence.
Acidity: take the aqueous solution under the item of clarity, and the pH value shall be 2.0 ~ 4.0 (appendix of Chinese Pharmacopoeia 1977 Edition).
It was determined by high performance liquid chromatography (Appendix VI D, Volume I, Chinese Pharmacopoeia, 2000 EDITION).
Octadecyl silane bonded silica gel was used as filler in chromatographic conditions and system applicability test; Methanol acetonitrile formic acid water (30:10:1:59) was the mobile phase; The detection wavelength was 286 nm. The number of theoretical plates calculated according to salvianolic acid B peak shall not be less than 2000.
Preparation of reference solution accurately weigh an appropriate amount of salvianolic acid B reference solution and add water to make it containing 10% per 1ml μ G solution.
Preparation of test solution take about 0.2g of the product, accurately weigh it, put it into a 50ml measuring bottle, add an appropriate amount of methanol, sonicate for 20 minutes, cool it, add water to the scale, shake it well, filter it, accurately measure 1ml of continuous filtrate, put it into a 25ml measuring bottle, add water to the scale, shake it well.
The determination method accurately absorbs 20% of the control solution and 20% of the test solution μ l. Inject it into the liquid chromatograph for determination.
Salvianolic acid B is the condensation of three molecules of Danshensu and one molecule of caffeic acid. It is one of the more studied salvianolic acids. It has important pharmacological effects on heart, brain, liver, kidney and other organs.
Salvianolic acid B has a strong antioxidant effect. Experiments in vivo and in vitro show that salvianolic acid B can scavenge oxygen free radicals and inhibit lipid peroxidation. Its action intensity is higher than that of vitamin C, vitamin E and mannitol. It is one of the known natural products with the strongest antioxidant effect Pharmacological studies show that salvianolic acid for injection has obvious antioxidant effect, inhibits platelet aggregation and thrombosis, and can prolong the survival time of animals under hypoxia. The results showed that salvianolic acid for injection (60 ~ 15mg / kg) could significantly improve the neurological deficit in rats with cerebral ischemia-reperfusion injury, improve behavioral disorder and significantly reduce the area of cerebral infarction. There was significant difference between high and medium doses (60 and 30mg / kg); Salvianolic acid for injection can significantly improve the neurological damage caused by FeCl3 induced cerebral ischemia in rats at 1, 2 and 24 hours after administration, which is manifested in the improvement of behavioral disorder and the reduction of cerebral infarction area; Salvianolic acid 40 mg / kg for injection significantly inhibited the aggregation of rabbit platelets induced by ADP, arachidonic acid and collagen, and the inhibition rates were 81.5%, 76.7% and 68.9% respectively. Salvianolic acid 60 and 30mg / kg for injection significantly inhibited thrombosis in rats; Salvianolic acid 60 and 30mg / kg for injection significantly prolonged the survival time of mice under hypoxia.
This product has the effects of promoting blood circulation and removing blood stasis, dredging meridians and activating collaterals. It is mainly used to treat ischemic stroke caused by blood stasis blocking meridians, such as numbness of half body and limbs, weakness, contracture pain, motor failure, mouth and eye deflection, etc.
In a cool and dry place.
Term of Validity
2-8 ° C, stored in a cool and dry place and away from light.
Matters Needing Attention
The product should be stored at low temperature. If it is exposed to air for a long time, the content will be reduced.