Verbascoside CAS No. 61276-17-3
Macluumaadka Muhiimka ah
[name]Mullein glycoside
[alias]ergosterol, Mullein
[Qaybta]phenylpropanoid glycosides
[Magaca Ingiriisiga]acteoside;Verbascoside;Kusaginin
[qaabka molecular]C29H36O15
[miisaanka unugyadu]624.59
[CAS Maya.]61276-17-3
Tilmaamaha Kiimikaad
[hantida]alaabtani waa budo crystal irbad cad
[cufnaanta qaraabada]1.6g/cm3
[lalan karo]si fudud ugu milmi kara ethanol, methanol iyo ethyl acetate.
Isha Soo saarista
Alaabtani waa jirrid hilib qallalan oo leh caleemo qolof leh oo ah Cistanche deserticola, oo ah warshad qoyska liedang ah.
Habka Imtixaanka
HPLC ≥ 98%
Xaaladaha Chromatographic: Wajiga mobilada methanol acetonitrile 1% acetic acid (15:10:75), heerka socodka 0.6 ml · min-1, heerkulka tiirka 30 ℃, hirarka dhererka 334 nm ( tixraac kaliya)
Shaqada iyo Isticmaalka
Alaabtan waxa loo isticmaalaa go'aaminta nuxurka
Habka Kaydinta
2-8 ° C, meel ka fog iftiinka.
Bioactivity ee Verbascoside
Barashada Vitro dhexdeeda:
Sida tartanka PKC Inhibitor ee ATP, Verbascoside waxay leedahay IC50 ee 25 μM Verbascoside (5.10) μ M
Barashada Vivo:
Verbascoside (1%) waxay hoos u dhigtay dhacdooyinka guud ahaan dabeecadda xoqitaanka iyo darnaanta nabarrada maqaarka ee qaabka jiirka ee 2,4-dinitrochlorobenzene (DNCB) - dermatitis atopic ah (AD).Verbascoside waxay sidoo kale xannibi kartaa pro-inflammatory cytokine TNF ee nabarrada maqaarka ee DNCB ay keentay - α, Muujinta IL-6 iyo IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) ma beddelin qabowga xanuunka aan caadiga ahayn ee uu keeno dhaawac dabadheeraad ah (CCI).Verbascoside (200 mg / kg, IP) waxay hoos u dhigtay xasaasiyadda qabow ee acetone maalintii 3. Verbascoside waxay sidoo kale si weyn hoos ugu dhigtay isbeddellada habdhaqanka ee la xidhiidha neuropathy.Intaa waxaa dheer, Verbascoside waxay hoos u dhigtay Bax waxayna kordhisay Bcl-2 maalinta 3 [3].
Tijaabada Unug:
Lymphocytic mouse leukemia L1210 unugyada (ATCC, CCL 219) waxay ka kooban yihiin 10% serum bovine uurjiifka, 4 mM glutamine, 100 U / ml penicillin, 100 μ Ml streptomycin sulfate iyo Verbascoside (ku milmay DMSO).Kobaca waxa lagu kormeeray iyadoo la tiriyay tirada unugyada ku jira miiska Coulter ka dib 2 maalmood oo ku-jiidhnimo jawi qoyan (5% CO2 ee hawada) at 37 ℃.Qiimaha IC50 waxaa la xisaabiyay iyadoo lagu salaynayo xariiqda dib-u-celinta tooska ah ee loo sameeyay xarun kasta oo tijaabo ah [1].
Tijaabada Xoolaha:
Si loo keeno atopic dermatitis (AD) - sida calaamadaha, jiirarka [2] waxay isticmaaleen 2,4-dinitrochlorobenzene (DNCB).Marka la soo koobo, timaha dhabarka ee jiirarka ayaa lagu saaray maqas elektaroonig ah 2 maalmood kahor daawaynta DNCB.Ma 200 μ L oo ah 1% DNCB (ku jirta acetone: saliid saytuun = 4:1) ayaa lagu marsiiyay maqaarka dambe ee xiiran si loo dareensiiyo.Weerarada soo noqnoqda ayaa lagu sameeyay isla goobta, 0.2% DNCB 3dii maalmoodba mar ilaa 2 toddobaad.Jiirarka ayaa loo qaybiyay 4 kooxood (n = 6 koox kasta): (1) gaadiidka lagu daaweeyay kantaroolka, (2) DNCB daweeyay kaliya, (3) 1% Verbascoside (acetone: saliid saytuun 4:1) - daweeyay kaliya, iyo ( 4) DNCB + 1% kooxda daaweeyay Verbascoside[2].
Tixraac:
[1]Herbert JM, iyo al.Verbascoside waxay ka go'day Lantana camara, horjoogaha borotiinka kinase C.J Nat Prod.1991 Nof-Dec;54(6):1595-600.
[2].Li Y, iyo al.Verbascoside waxay yaraynaysaa Atopic Dermatitis-ka Calaamadaha Jiirarka iyada oo loo sii marayo Saamaynta Ka-hortagga bararka ee Awoodda leh.Xasaasiyadda Int Arch Immunol.2018;175 (4):220-230.
[3]Amin B, iyo al.Saamaynta Verbascoside ee Xanuunka Neuropathic ee ay keentay Dhaawaca Cidhiidhiga ah ee Jiirka.Phytother Res.2016 Jan; 30 (1): 128-35.
