Verbascoside CAS No 61276-17-3
Amakuru y'ingenzi
[izina]Mullein glycoside
alias]ergosterol, Mullein
[icyiciro]fenylpropanoid glycoside
[Izina ry'icyongereza]acteoside;Verbascoside;Kusaginin
[molekulari]C29H36O15
uburemere bwa molekile]624.59
[CAS No.]61276-17-3
Ibyiza bya fiziki
[imitungo]iki gicuruzwa ni urushinge rwera rwa kirisiti
[ubucucike bugereranije]1.6g / cm3
[solubility]byoroshye gushonga muri Ethanol, methanol na Ethyl acetate.
Inkomoko yo gukuramo
Iki gicuruzwa nigiti cyumye cyumye gifite amababi manini ya Cistanche deserticola, igihingwa cyumuryango wa liedang.
Uburyo bwo Kwipimisha
HPLC ≥ 98%
Imiterere ya Chromatografiya: icyiciro cya mobile methanol acetonitrile 1% acide acetike (15:10:75), umuvuduko wa 0,6 ml · min-1, ubushyuhe bwinkingi 30 ℃, uburebure bwumurongo wa 334 nm (kubisobanura gusa)
Imikorere no Gukoresha
Iki gicuruzwa gikoreshwa mukugena ibirimo
Uburyo bwo Kubika
2-8 ° C, ibitswe kure y'umucyo.
Bioactivite ya Verbascoside
Mu Kwiga Vitro:
Nkurunziza PKC Inhibitor ya ATP, Verbascoside ifite IC50 ya 25 μ M。 Verbascoside yerekanye kis ya 22 na 28 ugereranije na ATP na histone, μ M。 Verbascoside ifite ibikorwa bya antitumor kuri selile L-1210 hamwe na IC50 ya 13 μ M .
Mu bushakashatsi bwa Vivo:
Verbascoside (1%) yagabanije kwandura muri rusange imyitwarire yubushuhe hamwe nuburemere bwibisebe byuruhu muburyo bwimbeba ya 2,4-dinitrochlorobenzene (DNCB) - iterwa na dermatite ya Atopic (AD).Verbascoside irashobora kandi guhagarika cytokine TNF itera inflammatory muri DNCB itera ibikomere byuruhu- α ， Kugaragaza IL-6 na IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) ntabwo yahinduye ububabare bukonje budasanzwe bwatewe no gukomeretsa bidakira (CCI).Verbascoside (200 mg / kg, IP) yagabanije allergie kuri acetone ikonje ikonje kumunsi wa 3. Verbascoside nayo yagabanije cyane impinduka zimyitwarire ijyanye na neuropathie.Byongeye kandi, Verbascoside yagabanije Bax yongera Bcl-2 kumunsi wa 3 [3].
Ubushakashatsi bwakagari:
lymphocytike imbeba leukemia L1210 selile (ATCC, CCL 219) yarimo 10% serumu ya bovine, 10 mM glutamine, 100 U / ml penisiline, 100 μ Ml streptomycine sulfate na Verbascoside (yasheshwe muri DMSO).Gukura byakurikiranwe no kubara umubare w'utugingo ngengabuzima twa Coulter nyuma y'iminsi 2 yubushakashatsi mu kirere cyuzuye (5% CO2 mu kirere) kuri 37 ℃.Agaciro IC50 yabazwe hashingiwe ku murongo wo gusubira inyuma washyizweho kuri buri kizamini [1].
Ubushakashatsi ku nyamaswa:
Mu rwego rwo gutera dermatite ya Atopic (AD) - nk'ibimenyetso, imbeba [2] yakoresheje 2,4-dinitrochlorobenzene (DNCB).Muri make, umusatsi wijimye wimbeba wakuweho numukasi wa elegitoronike iminsi 2 mbere yo kuvura DNCB.Uzaba 200 μ L ya 1% DNCB (muri acetone: amavuta ya elayo = 4: 1) yakoreshejwe kuruhu rwinyuma rwogosha kugirango akangurwe.Ibitero byagarutsweho byakorewe kurubuga rumwe, 0.2% DNCB buri minsi 3 mugihe cibyumweru 2.Imbeba zagabanyijwemo amatsinda 4 (n = 6 muri buri tsinda): (1) ibinyabiziga bivura, (2) DNCB ivurwa gusa, (3) 1% Verbascoside (acetone: amavuta ya elayo 4: 1) - ivurwa gusa, na ( 4) DNCB + 1% Itsinda rivura Verbascoside [2].
Reba:
[1].Herbert JM, n'abandi.Verbascoside yitandukanije na Lantana camara, ibuza protein kinase C. J Nat Prod.1991 Ugushyingo-Ukuboza; 54 (6): 1595-600.
[2].Li Y, n'abandi.Verbascoside igabanya ibimenyetso bya Atopic Dermatitis-Ibimenyetso nk'imbeba binyuze mu ngaruka zayo zo kurwanya indwara.Int Arch Allergie Immunol.2018; 175 (4): 220-230.
[3].Amin B, n'abandi.Ingaruka za Verbascoside mububabare bwa Neuropathique Yatewe no gukomeretsa karande mu mbeba.Phytother Res.2016 Mutarama; 30 (1): 128-35.
