Verbascoside CAS No. 61276-17-3
Ruzivo Rwakakosha
[zita]Mullein glycoside
[zvinoreva]ergosterol, Mullein
[chikamu]phenylpropanoid glycosides
[Zita rechirungu]acteoside;Verbascoside;Kusaginin
[molecular formula]C29H36O15
[molecular weight]624.59
[CAS Nha.]61276-17-3
Physicochemical Properties
[zvinhu]chigadzirwa ichi chena tsono crystal upfu
[relative density]1.6g/cm3
[kunyungudika]inonyungudika nyore mu ethanol, methanol uye ethyl acetate.
Extraction Source
Ichi chigadzirwa idzinde rakaomarara rine mashizha ane makwasha eCistanche deserticola, chirimwa chemhuri ye liedang.
Test Method
HPLC ≥ 98%
Chromatographic mamiriro: mobile phase methanol acetonitrile 1% acetic acid (15:10:75), flow rate 0.6 ml · min-1, column tembiricha 30 ℃, yekuona wavelength 334 nm (yekureva chete)
Basa uye Kushandisa
Ichi chigadzirwa chinoshandiswa kugadzirisa zvirimo
Kuchengeta Nzira
2-8 ° C, yakachengetwa kure nechiedza.
Bioactivity yeVerbascoside
In Vitro Chidzidzo:
Seanokwikwidza PKC Inhibitor yeATP, Verbascoside ine IC50 ye25 μ M [1]。 Verbascoside (5,10) μ M) Inhibition ye2,4-dinitrochlorobenzene (DNCB) - induced T cell costimulatory factors CD86 uye CD54, proinflammatory cytokines uye NF mu thk-1 masero κ B nzira activation [2].
MuVivo Zvidzidzo:
Verbascoside (1%) yakaderedza chiitiko chekukwenya kwese uye kuoma kwemaronda eganda mumhando yembeva ye2,4-dinitrochlorobenzene (DNCB) - induced atopic dermatitis (AD).Verbascoside inogonawo kuvhara pro-inflammatory cytokine TNF muDNCB inokonzera maronda eganda- α, Kuratidzwa kweIL-6 uye IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) haina kuchinja kutonhora kunorwadza kunokonzerwa nekukuvara kusingaperi (CCI).Verbascoside (200 mg / kg, IP) yakaderedza kusagadzikana kune inotonhora yakakurudzira acetone pazuva 3. Verbascoside yakaderedzawo zvakanyanya kuchinja kwemaitiro kunobatanidza neuropathy.Mukuwedzera, Verbascoside yakaderedza Bax uye yakawedzera Bcl-2 pazuva 3 [3].
Cell Experiment:
lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) ine 10% fetal bovine serum, 4 mM glutamine, 100 U/ml penicillin, 100 μ Muchikamu che24 chechikwata cheDulbecco chakagadziridzwa Eagle medium, masero 104 patsime akanga ari mashoma. Ml streptomycin sulfate uye Verbascoside (yakanyungudika muDMSO).Kukura kwakatariswa nekuverenga nhamba yemasero muCoulter counter mushure memazuva maviri ekuiswa mumhepo ine hunyoro (5% CO2 mumhepo) pa37 ℃.Iko IC50 kukosha kwakaverengerwa zvichienderana nemutsara wekudzoreredza mutsara wakatemerwa kune yega yega test compound [1].
Kuedza kwemhuka:
Kuti iite atopic dermatitis (AD) - sezviratidzo, makonzo [2] akashandisa 2,4-dinitrochlorobenzene (DNCB).Muchidimbu, vhudzi remusana wemakonzo rakabviswa nemagetsi scissors 2 mazuva asati aita DNCB kurapwa.Will 200 μ L ye1% DNCB (muacetone: mafuta omuorivhi = 4: 1) yakashandiswa kune ganda rekuseri rakaveurwa kuti rinzwe.Kurwiswa kwakadzokororwa kwakaitwa panzvimbo imwe chete, 0.2% DNCB mazuva ose e3 kwemavhiki maviri.Mice dzakakamurwa kuva 4 mapoka (n = 6 muboka rimwe nerimwe): (1) motokari yakabatwa kutonga, (2) DNCB inorapwa chete, (3) 1% Verbascoside (acetone: mafuta omuorivhi 4: 1) - inorapwa chete, uye ( 4) DNCB + 1% Verbascoside yakarapwa boka[2].
Reference:
[1].Herbert JM, nevamwe.Verbascoside yakaparadzaniswa kubva kuLantana camara, inhibitor yeprotein kinase C. J Nat Prod.1991 Nov-Dec;54(6):1595-600.
[2].Li Y, nevamwe.Verbascoside Inoderedza Atopic Dermatitis-Senge Zviratidzo muMbeva kuburikidza neIyo Yakasimba Anti-Inflammatory Effect.Int Arch Allergy Immunol.2018;175(4):220-230.
[3].Amin B, nevamwe.Mhedzisiro yeVerbascoside muNeuropathic Pain Inokonzerwa neChronic Constriction Injury mumakonzo.Phytother Res.2016 Jan;30(1):128-35.
