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Chinangwa:JNK1 jnk2 NF- κ B

In vitro kudzidza:isovitexin inodzivirira LPS yakakonzeresa oxidative kukuvara nekudzivisa kugadzirwa kweiyo intracellular ROS, uye zvakare inonetesa mhedzisiro yeH2O2 pane sero kushanda.Iine LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) yakanga isina cytotoxicity kune mbishi 264.7 masero, asi 200 μ G / ml isovitexin yakaratidza yakakosha cytotoxicity.Isovitexin (25,50) μ G / ml) inhibited LPS induced TNF- α, Kuwedzera mazinga e IL-6, iNOS uye COX-2.Isovitexin (25,50) μ G / ml) yakadzivisawo I mumasero matsvuku 264.7 κ B α Phosphorylation uye kuora mwoyo, iyo inopindirana nemigumisiro yeJNK1 / 2 inhibitor [1].

In vivo zvidzidzo:isovitexin (50 uye 100 mg / kg, IP) yakakonzera kushomeka kwakanyanya kwehistopathological shanduko muzvikamu zvemapapu uye yakaderedza huwandu hwemasero ekuzvimba muLPS mbeva.Heteroeosinophils (50 uye 100 mg / kg, IP) yakaderera TNF ne- α Uye IL-6 kugadzirwa, ROS kugadzirwa, MPO uye MDA zvinyorwa, yakawedzera SOD uye GSH, uye yakadzivirira LPS inokonzera kuzvimba uye oxidative kusagadzikana muLPS yakakonzera Ali mice.Uye zvinobudirira inhibisa mapuroteni kutaura kweNOS uye COX-2 [1].Isovitexin (25,50, 100 mg / kg) yakaderedza chiyero chekupona kweLPS / D-gal yakakonzera kukuvara kwechiropa mumakonzo nenzira inoenderana nedosi.Isovitexin yakadzivisawo NF- κ B uye kumusoro inoronga LPS / D-gal yakakonzera Nrf2 uye HO-1 mumakonzo [2].

Sero kuedza:Kushanda kwesero kwakatemwa neMTT assay.Raw 264.7 masero akaiswa pane 96 tsime mahwendefa (1) × 104 masero / zvakanaka) uye neakasiyana-siyana eisovitexin (yekupedzisira concentration: 0-200) μ G / ml) uye LPS (2 μ G / ml) kwemaawa makumi maviri nemana.Mukuwedzera, shandisa IV (25 kana 50 μ G / ml) pretreated masero kweawa imwe, uye ipapo H 2O 2 (300) yakawedzerwa μ M）。 Mushure memaawa makumi maviri nemana, MTT (5 mg / ml) yakawedzerwa kune macells ndokuzoiswa kwemaawa mana [1].

Kuedza kwemhuka:mbeva [1] kuitira kumisa Ali modhi, mbeva dzakakamurwa zvisina tsarukano mumapoka e6: control (saline), chete isovitexin (100 mg / kg, yakanyungudika mu0.5% DMSO), chete LPS (0.5 mg / kg, yakanyungudika mu saline ), LPS (0.5 mg / kg) + isovitexin (50 kana 100 mg / kg) uye LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, yakanyungudutswa mu saline).Isovitexin kana DEX (5 mg / kg) isovitexin yaipihwa.Mushure mekuratidzwa kune isovitexin kana DEX kweawa ye1, mbeva dzakabatwa anesthetized ne ether uye LPS yaishandiswa intranasally (mu) kuti iite kukuvara kwemapapu.Mhuka idzi dzakasunungurwa maawa gumi nemaviri mushure mekutonga kweLPS.Nokudaro, bronchoalveolar lavage fluid (BALF) uye sampuli dzemapapu emapapu zvakakohwewa kuyera mazinga e cytokine;ROS chizvarwa;SOD, GSH, MDA uye MPO mabasa;Uye kutaura kweCOX-2, iNOS, HO-1 uye Nrf2 mapuroteni [1].