Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Kenyelletso e Khutšoanyane
Glycyrrhizin, e tsejoang hape e le Liquiritin.Licorice ke semela sa Glycyrrhiza ho Leguminosae.Metso le likutu tsa eona ke litlama tse tloaelehileng tsa Sechaena.
Meriana e ajoa haholo ka leboea-bochabela ho China, Xinjiang, Yunnan, Inner Mongolia, Anhui le libakeng tse ling.Shennong materia medica classic e e thathamisa e le ea boemo bo holimo, e re " joang bona ke morena oa meriana eohle, 'me ke ba seng bakae ba sa bo sebeliseng".Licorice e na le likarolo tse rarahaneng, haholo-holo ho kenyelletsa triterpenoids, flavonoids le coumarin.Flavonoids ke mofuta oa metsoako ea bioactive e fumanoang ho tsoa ho licorice extract.Likarolo tsa eona tsa lik'hemik'hale tsa meriana haholo-holo li kenyelletsa glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, joalo-joalo Lilemong tsa morao tjena, ho bile le litlaleho tsa ho senya mahala, antioxidant, anti-cancer le anti mutagenic liphello tsa licorice flavonoids lapeng le linaheng tse ling.
Lik'hemik'haleName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPthepa:Monohydrate (hlapolla ethanol kapa metsi), ntlha e qhibilihang: 212 ~ 213 ° ℃.
Ketso ea Pharmacological
Chefo: Ha ho letho
Boitšoaro bo bobe: ha bo tsejoe
Mohloli oa motsoako: legume Glycyrrhiza glabra L. motso, Glycyrrhiza uralensis Fisch Root.
Ho tlosoa ha Glycyrrhizin
Phekolo ea Licorice Raw Materials
Motsoako oa lik'hemik'hale oa thepa e tala ea licorice e rarahane haholo.E le ho fumana phello e ntle ea karohano, fokotsa tšilafalo ea litšila holim'a kholomo ea chromatographic, le ho ntlafatsa litaba tsa glycyrrhizin ka thepa e tala ea ente, mokhoa oa ho ntša o ile oa sebelisoa ho lokisa thepa e tala.Beha furu ea 4G ka tekanyo ea elektronike 'me ue kenye ka beaker.Metha ka nepo 100ml ea metsi a qhibilihisitsoeng ka silindara ea ho lekanya ebe u e tšela ka bekeng bakeng sa ho qhibiliha.Ultrasonic bakeng sa metsotso e ka bang 15, 'me kamehla u tsose ka molamu oa khalase ho potlakisa ho qhibiliha.Ebe u kenya beaker ka bateng ea 90 ℃ ea thermostatic 'me ue futhumale lihora tse 2, ebe ue futhumatsa hore e sefa.Ka mor'a ho kenya filtrate ka n-butanol solvent le ho ema metsotso e 'maloa, boholo ba li-glycosides li qhibiliha ka n-butanol solvent, ebe li etsa tlhahiso ea bobeli, li ntša palo e nyenyane ea glycosides e setseng ka metsing, ebe qetellong e kopanya le ho tsepamisa mohopolo oa n- tharollo ea butanol e fumanoeng ka ho ntšoa ha bobeli bakeng sa chromatography le tlhoekiso.
Tlhoekiso ea Glycyrrhizin Ka Chromatography
Nka 10 ml ea sehlahisoa se ntšitsoeng ka holimo e le lisebelisoa tse tala, qala pompo, beha sekhahla sa phallo ho 25 ml / min, 'me u tlise thepa e tala ka 500 mm ka mohato oa mohala (methanol: metsi = 1: 4) × Limilimithara tse 40 tsa ho itokisa, bokella karoloana ea furu ea glucoside sehlahisoa ho ea ka boemo ba tlhōrō: karoloana ea 1 h ea pele e bokelloa hammoho e le karoloana ea pele ho litšila, ebe e fetola phallo.Mohlala, hlatsoa kholomo ka motsoako oa 50% ea methanol le metsi, kopanya sehlahisoa metsotso e meng le e meng e 20, ebe u tsepamisa botlolo e 'ngoe le e' ngoe ea sehlahisoa ka mouoane oa rotary, 'me u nke 20 µ L bakeng sa tlhahlobo ea chromatographic ea HPLC, Ho fihlela ho sa fumanoe sepheo.Maemo a ho lemoha a HPLC a ne a le a latelang: mohato oa selefouno: methanol: metsi = 3.5: 6.5;Mokhahlelo oa ho emisa: silika gel carbon 18;Kholomo ea Chromatographic: 450 mm × 4.6 mm; Sekhahla sa phallo: 1 ml / min;Nako ea ho lemoha: 254nm.Likahare tsa glycyrrhizin ka botlolo ea bobeli ke tsona tse phahameng ka ho fetisisa har'a lihlahisoa tse fumanoang metsotso e meng le e meng ea 20
Tlhoekiso ea Glycyrrhizin Ka Rechromatography
Kaha litaba tsa glycyrrhizin ka mor'a tlhoekiso ea mantlha ea chromatographic ha li phahame, ho khethoa mokhoa o tšoanang.Nka 10 ml ea sehlahisoa se hloekisitsoeng ka holimo e le thepa e tala e emeng, sekhahla sa phallo ke 25 ml / min, 'me u tlise botlolo ea bobeli ea sehlahisoa ho 500 mm ka mohato oa mohala (methanol: metsi = 2: 5) × Ka 20 mm. } chromatographic column, bokella distillate ea furu glycoside sehlahisoa ho ea ka boemo ba tlhoro: hokela sehlahisoa metsotso e meng le e meng e 4, ebe u tsepamisa botlolo e 'ngoe le e' ngoe ea sehlahisoa ka mouoane oa rotary, 'me u sebelise mohala o tšoanang oa ho lemoha ka holimo bakeng sa tlhahlobo ea chromatographic ea HPLC ho fihlela ho se na sepheo. .Ka mor'a tlhahlobo, ho ile ha fumanoa hore litaba tsa glycyrrhizin ka botlolo ea botšelela e ne e le tse phahameng ka ho fetisisa har'a lihlahisoa tse fumanoang metsotso e meng le e meng ea 4, moo nako ea ho boloka e neng e le 5.898 min e le tlhōrō ea sepheo, 'me litaba li fihlile hoo e ka bang 40% ka mokhoa oa ho tloaeleha sebakeng. .
Post Phekolo Of Products
Sehlahisoa se bokeletsoeng se tšeloa tlas'a khatello e fokotsehileng holim'a mouoane oa rotary ka 70 ℃.Ka mor'a hore solvent e fetohe mouoane, qhala sehlahisoa se tiileng holim'a botlolo e pota-potileng ka tekanyo e nyenyane ea methanol, 'me u khanye ka har'a tube ea teko mocheso oa kamore ho fihlela likristale tse tšoeu tsa granular li hlaha [2].
