Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Sumo Pfupi
Glycyrrhizin, inozivikanwawo seLiquiritin.Licorice chirimwa cheGlycyrrhiza muLeguminosae.Midzi yaro uye madzinde ndiwo akajairika maChinese herbs.
Mushonga unoparadzirwa zvakanyanya muNortheast China, Xinjiang, Yunnan, Inner Mongolia, Anhui nedzimwe nzvimbo.Shennong materia medica classic inonyora segiredhi repamusoro, ichiti "uswa uhu ndihwo mambo wemishonga yese, uye kune vashoma vasingahushandise".Licorice ine zvikamu zvakaoma, kunyanya kusanganisira triterpenoids, flavonoids uye coumarins.Flavonoids imhando yezvinyorwa zvebioactive zvakatorwa kubva ku licorice extract.Mishonga yaro yemakemikari yemishonga inonyanya kusanganisira glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, etc. Mumakore achangopfuura, kune mishumo pamusoro pekusununguka kwemahara, antioxidant, anti-cancer uye anti mutagenic migumisiro ye licorice flavonoids kumba uye kune dzimwe nyika.
ChemicalName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPdenga:Monohydrate (dilute ethanol kana mvura), nzvimbo yakanyunguduka: 212 ~ 213 ° ℃.
Pharmacological Action
Toxicity: Hapana
Kuita kwakashata: hazvizivikanwe
Ingredient source: legume Glycyrrhiza glabra L. mudzi, Glycyrrhiza uralensis Fisch Root.
Kubviswa kweGlycyrrhizin
Pretreatment yeLicorice Raw Materials
Iyo kemikari inoumbwa ye licorice raw material yakaoma kwazvo.Kuti uwane kupatsanurwa kuri nani, kuderedza kusvibiswa kwetsvina pane yekugadzirira chromatographic column, uye kugadzirisa zvirimo zve glycyrrhizin mune jekiseni raw material, nzira yekubvisa yakashandiswa pretreat iyo mbishi.Weresa 4G uswa nechiyero chemagetsi woisa mubeaker.Nyatsoyera 100ml mvura yakagayiwa necylinder yekuyeresa woidira mubeaker kuti ipere.Ultrasonic kwemaminetsi gumi nemashanu, uye gara uchimutsa negirazi tsvimbo kuti ikurumidze kuparara.Wobva waisa beaker mune 90 ℃ thermostatic bath woipisa kwemaawa maviri, wozoipisa kuti isefa.Mushure mekuwedzera iyo filtrate mu n-butanol solvent uye kumira kwemaminetsi akati wandei, mazhinji e glycosides anonyungudika mu n-butanol solvent, obva aita chidimbu chechipiri, anobvisa chidimbu cheglycosides akasara mumvura, uye pakupedzisira anosanganisa uye kuisa pfungwa pa n- butanol mhinduro yakawanikwa neyechipiri kutorwa kwechromatography uye kucheneswa.
Kucheneswa KweGlycyrrhizin By Chromatography
Tora 10 ml yechigadzirwa chakabudiswa pamusoro sechinhu chakasara, tanga pombi, isa mwero wekuyerera pa25 ml / min, uye uunze iyo mbishi mu500 mm nechikamu chenhare (methanol: mvura = 1: 4) × 40 mm yekugadzirira column, unganidza chikamu cheuswa glucoside chigadzirwa maererano nepamusoro pemamiriro ezvinhu: chikamu chekutanga 1 h chinounganidzwa pamwe chete sechikamu chekare chekusachena, uye zvino shandura kuyerera.Semuyenzaniso, geza koramu nemusanganiswa we50% methanol nemvura, batanidza chigadzirwa chega chega makumi maviri maminetsi, wobva waisa bhodhoro rega rega rechigadzirwa nekushanduka kwemhepo, uye tora makumi maviri µ L yeHPLC chromatographic ongororo, Kusvikira pasina chinangwa chaonekwa.Mamiriro ekuona eHPLC aive anotevera: mobile phase: methanol: mvura = 3.5: 6.5;Kumira chikamu: silica gel kabhoni 18;Chromatographic column: 450 mm × 4.6 mm; Kuyerera: 1 ml / min;Kuonekwa wavelength: 254nm.Zviri mukati me glycyrrhizin mubhodhoro rechipiri ndiyo yakakwirira pakati pezvigadzirwa zvakagamuchirwa maminitsi makumi maviri
Kucheneswa KweGlycyrrhizin By Rechromatography
Sezvo zvinyorwa zve glycyrrhizin mushure mekunatswa kwechromatographic hazvina kukwirira, nzira imwechete inosarudzwa.Tora 10 ml yechigadzirwa chepamusoro chakacheneswa seyakamisikidzwa, chiyero chekuyerera i25 ml / min, uye uunze bhodhoro rechipiri rechigadzirwa mu500 mm nechikamu chenhare (methanol: mvura = 2: 5) × Mu20 mm. } chromatographic column, unganidza distillate yehuswa glycoside chigadzirwa zvinoenderana nepamusoro pemamiriro ezvinhu: batanidza chigadzirwa chega chega 4 min, wobva waisa bhodhoro rega rechigadzirwa nekupoterera kwekutenderera, uye shandisa iyo yakafanana yekuona tambo kumusoro kweHPLC chromatographic ongororo kusvika pasisina chinangwa. .Mushure mekuongorora, zvakaonekwa kuti zviri mukati me glycyrrhizin mubhodhoro rechitanhatu ndiyo yaive yepamusoro-soro pakati pezvigadzirwa zvakagamuchirwa yega yega 4 min, umo nguva yekuchengeta yaive 5.898 min seyakanyanya kutariswa, uye zvirimo zvakasvika nezve 40% nenzira yenzvimbo yekugara. .
Post Kurapa KweZvigadzirwa
Chigadzirwa chakaunganidzwa chinodzikiswa pasi pekumanikidzwa kwakaderedzwa pane inotenderera evaporator pa70 ℃.Mushure mekunge chinonyungudutsa chanyungudika, nyungudutsa chigadzirwa chakasimba pafurasiki yepasi nekashoma kemethanol, uye penya muchubhu yekuyedza patembiricha yekamuri kusvika machena makristasi aonekwa [2].
