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Typhonine glycoside

Puhuang ndiyo mukume wakaomeswa weTypha angustifolia L., Typha Orientalis Presl kana zvirimwa zvakafanana muTyphaceae.Izvo zvirimo zveisorhamnetin-3-o-neohesperidin muChinese Pharmacopoeia (2005 Edition) inotemerwa neHPLC.Nzira huru dzekugadziriswa kweisorhamnetin-3-o-neohesperidin muTyphae uye magadzirirwo ayo

HPLC.Pasi pekugadzwa kwemukati kweTypha muChinese Pharmacopoeia (2005 Edition):

Chromatographic mamiriro uye system applicability bvunzo: octadecyl silane bonded silica gel sekuzadza;Mvura yeAcetonitrile (15:85) yakashandiswa sechikamu chekufambisa;Iyo yekuona wavelength yaive 254 nm.Column tembiricha 40 ℃.Nhamba yemahwendefa ezvinyorwa haifaniri kunge iri pasi pe1000 maererano nepamusoro-soro ye isorhamnetin-3-o-neohesperidin.

Kugadzirira kwebvunzo mhinduro: tora inenge 0.5g yechigadzirwa ichi, uiremedze nemazvo, uise mubhodhoro rekuyeresa 50ml, wedzera 45ml yemethanol, ultrasonic kurapwa (simba 250W, frequency 20KHz) kwemaminetsi makumi matatu, itonhodze, wedzera methanol kune chikero, zunza zvakanaka, sefa, uye tora inoenderera filtrate

Kutsunga kweisorhamnetin-3-o-neohesperidin muPuhuang uye gadziriro dzayo neHPLC.Iyo general mobile phase system ndeye acetonitrile mvura:

① Hp1050 yakakwira performance liquid chromatograph;Chromatographic column C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);Nharembozha: mvura yeacetonitrile (15:85);Kuonekwa wavelength: 254nm;Column greenhouse tembiricha.

② Iyo yakanyanya kuita yemvura chromatograph ndeye hp1050 system yeHewlett Packard Kambani muUnited States;Chromatographic column bondclone 10 C18 (3.9mm) × 300mm）; Nharembozha: mvura acetonitrile (80:20);yekuona wavelength 254 nm, bandwidth 4 nm;referensi wavelength 550 nm, bandwidth 100 nm;kudzvinyirirwa 8.

Fu Daxu et al.Yakasarudza zviri mukati me typhoniflorin uye isorhamnetin-3-o-neohesperidin mune yakazara flavone yakatorwa yeTypha neHPLC uye 1100 chromatograph.The chromatographic column is eclipse XdB C18 (250mm × 4.6mm，5 μ m); Nharembozha: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Iyo yekuona wavelength ndeye 248 nm.Kugadzirira kwese flavonoids yakabudiswa yeTypha: 50g yeTypha yakatorwa ne 60% ethanol (500ml) × 2), 1H nguva imwe neimwe, iyo yakasvibiswa yakasimbiswa pa40 ℃, iyo yakasvibiswa yakanyungudutswa ne 600ml mvura uye centrifuged.Iyo yakasara yakanyungudutswa ne300ml yemvura uye centrifuged.Sanganisa iyo centrifugal solution, gadzirisa huwandu kusvika 1000ml nemvura, ishambadze pambiru ye macroporous resin (150ml macroporous resin yakaoma uremu 75g) ne 1.5 times the crude drug amount, elute ne 600ml yemvura uye 450ml ye20% kubvisa ethanol tsvina, zvino elute ne 600ml ye 50% ethanol, uye iyo eluent inowanikwa ne vacuum drying pa 40 ℃.

Yang Yonghua et al.Yakashandiswa yakakwira performance capillary electrophoresis uye yakakwira performance liquid chromatography kuona zviri mukati meisorhamnetin 232o2, neohesperidin uye typhoside muPuhuang.Hpce-3d high performance capillary electrophoresis chiridzwa (Hewlett Packard, USA), quartz capillary column 50 μ m × 56cm (HP), lc-6a high performance liquid chromatograph (Shimadzu kambani yeJapan), C18 column 5mm × 200mm, particle size 5 μ M (kambani yeDalian Yite).HPCE mamiriro: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile ye sodium dodecyl sulfate (SDS), voltage 20kV, column tembiricha 30 ℃, yekuona wavelength 270nm, jekiseni vhoriyamu 30KPa · s.HPLC mamiriro: iyo mobile phase yaive acetonitrile mvura (15:85), iyo column tembiricha yaive 25 ℃, uye yekuona wavelength yaive 254 nm.

Typhaneoside Reference chinhu

[zita]typhonioside [1]

[CAS Nha.]104472-68-6

[nzira yekuona]HPLC ≥ 98%

[Mashoko]20mg 50mg 100mg 500mg 1g (inogona kuiswa mukati maererano nezvinodiwa nevatengi)

[zvinhu]amorphous powder