Imikhiqizo

Incazelo:
I-prim-o-glucosylcimifugin inemiphumela enamandla yokulwa nokuvuvukala.Inkulumo ye-iNOS ne-COX-2 ingavinjelwa ngokulawula isignali ye-JAK2 / STAT3

Izigaba ezifanele:
Indlela yokubonisa > > ukungatheleleki nokuvuvukala > > i-nitric oxide synthase
Inkambu yocwaningo > > ukuvuvukala / ukungatheleleki
Imikhiqizo Yemvelo > > abanye

Ithagethi:I-iNOS
I-COX-2

Isifundo se-In Vitro:
I-prim-o-glucosylglycine (POG) iwukuqukethwe okuphezulu kakhulu kwekhromone kanye nengxenye eyinhloko esebenzayo ku-Fangfeng (RS).I-Prim-o-glucosylglycine idlala indima yokulwa nokuvuvukala kuma-macrophage angakahluzwa angama-264.7 ngokuvimbela ukudluliselwa kwesignali ye-JAK2 / STAT3 futhi ivimbele ukuvezwa kwe-iNOS ne-COX-2.I-cytotoxicity ye-prim-o-glucoside ku-LPS eyenziwe yasebenza eluhlaza engama-macrophage angu-264.7 ikaliwe.Sebenzisa i-LPS (1 μ G / ml) nokwandisa ukugxila kwe-prim-o-glucosylglycine (15, 50 kanye no-100 μ G / ml) amahora angu-24, futhi ukusebenza kwamaseli kwahlolwa ngokuhlolwa kwe-CCK-8.Uma kuqhathaniswa namaseli aphathwe i-DMSO (ukulawula), amahora angu-24 kanye nokuchayeka ku-15-100 μ Ngemva kwe-g/ml prim-o-glucoside, ukusebenza kweseli akuzange kuthinteke kakhulu.Ukuze kufundwe umphumela wokunqanda ukuvuvukala we-prim-o-glucosylglycine, ukuthi i-prim-o-glucosylglycine ingaba nomthelela AKUKHO yini ukuhlanganiswa kwahlolwa kumaseli e-LPS acushiwe angu-264.7.Sebenzisa i-LPS (1 μ G / ml) kanye nokugxila okuhlukahlukene kwe-prim-o-glucosylglycine (15, 50 kanye no-100 μ G / ml) amahora angu-24.Ukugxilisa ingqondo akuzange kukalwe kumasiko angaphezu kwamandla ngokusabela kukaGriess.Ukugxiliswa kwe-cha ku-supernatant yesiko kukhuphuke kakhulu ngokuchayeka kwe-LPS, futhi i-prim-o-glucoside evimbe kakhulu i-LPS ayizange ikhiqize ukukhiqizwa ngendlela encike ekugxilweni [1].
Esifundweni se-Vivo
I-bronchoalveolar lavage fluid (BALF) iqoqwe emahoreni angu-7 ngemva kokuphathwa kwe-lipopolysaccharide (LPS), futhi izinga le-cytokine ku-BALF likalwa nge-ELISA.Uma kuqhathaniswa neqembu lokulawula, i-TNF ku-BALF- α, IL-1 β Futhi amazinga e-IL-6 anda kakhulu.Kodwa-ke, ukwelashwa kwangaphambili nge-prime-o-glucosylglycine (2.5, 5 noma 10 mg / kg) kwehlisa kakhulu i-TNF- α, IL-1 β Kanye ne-IL-6 ngendlela encike kumthamo (P <0.05, P <0.05, 0.01 [1].

Ukuhlolwa Kweseli
Ikhithi yokubala yamaseli (CCK-8) yasetshenziselwa ukunquma ukugcwala kwe-cytotoxic ye-prim-o-glucosylglycine.Ngamafuphi, amangqamuzana angama-264.7 aluhlaza aphathwe nge-1 emthonjeni ngamunye × Ubuningi bamaseli angu-104 afakwe emithonjeni ye-96 futhi afakwe ubusuku bonke.Bese sebenzisa 1 μ Amaseli avuselelwa nge-g/ml LPS futhi aphathwa ngokugxilisa okuhlukahlukene kwe-prim-o-glucosylglycine (15, 50 kanye no-100 μ g/mL; i-Medchem express, Princeton, NJ, USA) noma ukwelashwa kwe-DMSO.Ngemva kokufukamela ku-37 ℃ amahora angu-24, isisombululo se-CCK-8 sanezelwa emthonjeni ngamunye futhi sifakwa elinye ihora.I-Absorbance ikalwa ku-450 nm kusetshenziswa isifundi se-microplate [1].

Ukuhlolwa Kwezilwane
amagundane [1] BALB / C male amagundane, anamaviki angu-8 ubudala, anesisindo esingaba ngu-18 kuya ku-20g.Amagundane ahlukaniswa ngokungahleliwe abe amaqembu e-5: iqembu lokulawula;Iqembu le-LPS;I-LPS + prime-o-glucosylglycine (2.5, 5 noma 10mg / kg isisindo somzimba).I-Prime-o-glucosylglycine ilawulwa ngaphakathi kwe-intraperitoneally.Ngemuva kwehora elingu-1, amagundane eqembu le-LPS neqembu le-LPS + prime-o-glucosylglycine anikezwa ama-50 mg / L intranasal (in) (200 mg / L) μ LLPs ukuze enze ukulimala okukhulu kwamaphaphu.Amagundane okulawula anikezwe u-50% we-intranasal (in) ngaphandle kwe-LPS μ LPBS
[1]

Ireferensi:
[1].UZhou J, et al.I-Prim-O-glucosylcimifugin Inciphisa i-Lipopolysaccharide Response Evuvukalayo ku-RAW 264.7 Macrophages.I-Pharmacogn Mag.2017 Jul-Sep;13(51):378-384.
[2].Chen N, et al.I-Prime-O-glucosylcimifugin inciphisa ukulimala kwamaphaphu okubangelwa i-lipopolysaccharide kumagundane.I-Immunopharmacol.2013 Jun;16(2):139-47.