Galangin CAS No. 548-83-4
Iimpawu zomzimba kunye neekhemikhali
Isifaniso:iGaoliang Curcumin;3,5,7-trihydroxyflavone,
Igama lesiNgesi:galangin,
Igama lesiNgesi:3,5,7-trihydroxyflavone;3,5,7-trihydroxy-2-phenylchromen-4-enye
Ulwakhiwo lweemolekyuli
1. Isalathisi se-Molar refractive: 69.55
2. Umthamo weMolar (m3 / mol): 171.1
3. Umthamo othile we-Isotonic (90.2k): 519.4
4. Uxinzelelo lomphezulu (dyne / cm): 84.9
5. I-polarizability (10-24cm3): 27.57
I-Computational Chemistry
1. Ixabiso lokubhekisela kwi-hydrophobic parameter calculation (xlogp): Akukho
2. Inani labaxhasi be-hydrogen bond: 3
3. Inani le-hydrogen bond receptors: 5
4. Inani leebhondi zeekhemikhali ezijikelezayo: 1
5. Inani leetautomers: 24
6. Ummandla wepolarity yemolekyuli ye-topological 87
7. Inani leeathom ezinzima: 20
8. Intlawulo yomphezulu: 0
9. Ukuntsonkotha: 424
10. Inani le-athomu ze-isotopic: 0
11. Qinisekisa inani le-atomic stereocenters: 0
12. Inani leestereocenters zeathom ezingaqinisekanga: 0
13. Qinisekisa inani leekhemikhali bond stereocenters: 0
14. Inani lee-stereocenters zekhemikhali ezingapheliyo: 0
15. Inani leeyunithi zebhondi ye-covalent: 1
Isenzo se-Pharmacological
I-Galangin inokuguqula i-Salmonella typhimurium TA98 kunye ne-TA100 kwaye ibe nefuthe le-antiviral
Isifundo kwiVitro
UGalangin uthintele i-catabolism ye-DMBA ngendlela exhomekeke kumthamo.I-Galangin iphinde yanqanda ukubunjwa kwe-DMBA-DNA adducts kwaye ithintele i-DMBA eyenza inhibition yokukhula kweeseli.Kwiiseli ezingaguqukiyo kunye ne-microsomes ezihlukanisiwe kwiiseli eziphathwayo ze-DMBA, i-galangin ivelise inhibition esebenzayo exhomekeke kwidosi ye-CYP1A1 umsebenzi olinganiswa ngumsebenzi we-ethoxypurine-o-deacetylase.Uhlalutyo lwe-inhibition kinetics nge-double reciprocal diagram ibonise ukuba i-galangin inhibited CYP1A1 umsebenzi ngendlela engakhuphisani.I-Galangin ikhokelela ekunyuseni kwenqanaba le-CYP1A1 mRNA, ebonisa ukuba ingaba yi-agonist ye-aromatic hydrocarbon receptor, kodwa inqanda i-CYP1A1 mRNA (TCDD) eyenziwa yi-DMBA okanye i-2,3,5,7-tetrachlorodibenzo-p-dioxin.I-Galangin iphinda ivimbele i-DMBA okanye i-TCDD eyenziwe ukukhutshelwa kwe-reporter vectors equkethe i-CYP1A1 umgqugquzeli [1].Unyango lwe-Galangin luthintele ukwanda kweeseli kunye ne-autophagy (130) μ M) Kwaye i-apoptosis (370 μ M) I-3, kunye (3) ipesenti yokwandisa iiseli ezine-vacuoles.Intetho ye-P53 nayo yanda.I-Galangin induced autophagy yancitshiswa ngokuthintela i-p53 kwiiseli ze-HepG2, kunye nokugqithiswa kwe-p53 kwiiseli ze-Hep3B kubuyisele ipesenti ephezulu yeeseli ze-vacuoles ezibangelwa yi-galangin kumanqanaba aqhelekileyo. [2].
Uvavanyo lweSeli
Iiseli (i-5.0 × 103) zifakwe kwaye ziphathwe ngeendlela ezahlukeneyo ze-galangin kwiiplate ze-96 zamaxesha ahlukeneyo.Ngokudibanisa i-10 μ L ye-5 mg / ml isisombululo se-MTT ukumisela inani leeseli eziphilayo kwiqula ngalinye.Emva kokufakwa kwi-37 ℃ kwiiyure ze-4, iiseli zachithwa kwisisombululo se-100% esiqulethe i-20% ye-SDS kunye ne-50% ye-dimethylformamide μ L isisombululo.Ubuninzi be-optical density bubalwe kusetyenziswa i-varioskan flash reader spectrophotometer kwi-wavelength yovavanyo ye-570 nm kunye ne-reference wavelength ye-630 nm.
