Kayayyaki

Manufar:GABA receptor, mTOR, PI3K, Akt [1] [2]

Nazarin in vitro:ƙananan kashi 41 μ Jujuboside A na M (kimanin 0.05 g / L) haifar da GABA (a) masu karɓa a lokacin 24 da 72 h jiyya α 1, α 5, β 2 subunit mRNA ya karu sosai.Babban kashi 82 μM (kimanin 0.1 g / L) Jujuboside A ya ƙara yawan GABA (a) masu karɓa a 24 h α 1, α 5 matakan mRNA da rage β 2 mRNA matakin kuma rage GABA (a) receptor subunit α 1, β 2 mRNA magana an bi da shi a 72 h [1].Jujuboside A pretreatment na iya juyar da raguwar yuwuwar tantanin halitta kuma mafi kyawun haifar da rauni na kwayar H9c2 na ISO.Jujuboside A na iya hanzarta phosphorylation na PI3K, Akt da mTOR.Jujuboside A na iya rage yawan adadin furotin da ke da alaƙa da microtubule LC3-II / I a cikin ƙwayoyin H9c2 [2]

Nazarin vivo:a cikin rana (9:00-15:00), jujubosides sun ƙara yawan barcin barci da saurin motsin ido (REM), amma ba su da wani tasiri mai mahimmanci akan barcin REM (NREM).Da daddare (21:00-3:00), jujubosides sun ƙara yawan barcin barci da barcin NREM, musamman barci mai sauƙi, amma ba su da wani tasiri mai mahimmanci akan barcin REM da jinkirin barci (SWS) [3].Jiyya na intraventricular tare da jjuboside a an rage shi sosai ta hanyar Y-maze, gujewa aiki da ma'aunin ma'aunin ruwa na Morris β 1-42 ya haifar da koyo da raunin ƙwaƙwalwar ajiya a cikin mice.Intracerebroventricular allura na Jujuboside A rage hippocampal a β 1-42 matakan da muhimmanci hana ayyukan acetylcholinesterase (AChE) da a'a, da kuma rage yawan abun ciki na malondialdehyde (MDA) a cikin hippocampus da cerebral bawo na berayen allura zuwa lateral ventricle β 1-42. [4].

Gwajin kwayar halitta:bayan kwanaki 7 na al'ada a cikin vitro, ƙwayoyin suna ci gaba da fallasa su har tsawon sa'o'i 24 ko 72, kuma ba a fallasa su ga matsakaicin da ke dauke da Jujuboside A ko diazepam ko duka biyun.An ƙara Vector zuwa ƙungiyar kulawa;Ƙungiyar Diazepam ta ƙara 10 μ M diazepam;Jujuboside A82 μM (kimanin 0.1g / L) da 41 μM (kimanin 0.05 g / L) an ƙara su zuwa ƙungiyoyin jua-h (babban kashi Jujuboside A) da jua-l (ƙananan kashi Jujuboside A), bi da bi.An keɓe jimlar RNA daga sel don ƙarin bincike [1].

Gwajin dabba:beraye: an yi allurar ta ICV β 1-42 ta haifar da rashin fahimta a cikin mice.Sannan, an yi wa berayen allurar Jujuboside A (0.02 da 0.2 mg/kg) na intraventricular (ICV) na tsawon kwanaki 5.Y maze, gujewa aiki da kuma gwajin maze na Morris akan beraye [1].