Zogulitsa

Zolinga:GABA cholandilira, mTOR, PI3K, Akt [1] [2]

Maphunziro a in vitro:mlingo wochepa 41 μ Jujuboside A wa M (pafupifupi 0.05 g / L) anachititsa GABA (a) zolandilira pa 24 ndi 72 h mankhwala α 1, α 5, β 2 subunit mRNA inawonjezeka kwambiri.Mlingo waukulu 82 μ M (pafupifupi 0.1 g / L) Jujuboside A yowonjezera kwambiri GABA (a) zolandilira pa 24 h α 1, α 5 subunit mRNA milingo ndi kuchepa kwa β 2 subunit mRNA mlingo ndi kuchepa GABA (a) receptor subunit α 1, β 2 mRNA mawu adachitidwa pa 72 h [1].Jujuboside A pretreatment imatha kusintha kuchepa kwa maselo ndikupangitsa kuti ISO ipangitse H9c2 kuvulala kwama cell.Jujuboside A imatha kufulumizitsa phosphorylation ya PI3K, Akt ndi mTOR.Jujuboside A imatha kuchepetsa kwambiri kuchuluka kwa mapuloteni ogwirizana ndi microtubule LC3-II / I m'maselo a H9c2 [2]

Maphunziro a vivo:masana (9: 00-15: 00), jujubosides anawonjezera kwambiri kugona kwathunthu ndi kuyenda kwa maso mofulumira (REM), koma sizinakhudze kwambiri kugona kwa non REM (NREM).Usiku (21:00-3:00), ma jujubosides amachulukitsa kwambiri kugona kwathunthu ndi kugona kwa NREM, makamaka kugona pang'ono, koma sikunakhudze kwambiri kugona kwa REM ndi kugona pang'onopang'ono (SWS) [3].Chithandizo cha intraventricular ndi jjuboside a chinachepetsedwa kwambiri kudzera mu Y-maze, kupewa mwachangu komanso miyeso ya maze ya Morris madzi β 1-42 yochititsa kuphunzira ndi kuwonongeka kwa kukumbukira mu mbewa.Jekeseni wa intracerebroventricular wa Jujuboside A kuchepa kwa hippocampal ndi β 1-42 milingo inalepheretsa kwambiri ntchito za acetylcholinesterase (AChE) ndi ayi, ndipo inachepetsa kuchuluka kwa malondialdehyde (MDA) mu hippocampus ndi cerebral cortex ya mbewa zojambulidwa mu lateral β1 ventricle 2 lateral. [4].

Kuyesera kwa ma cell:pambuyo pa masiku a 7 a chikhalidwe cha mu vitro, maselowa adawonekera mosalekeza kwa maola 24 kapena maola 72, ndipo sanawonekere ku sing'anga yomwe ili ndi Jujuboside A kapena diazepam kapena zonse ziwiri.Vector adawonjezeredwa ku gulu lolamulira;Gulu la Diazepam linawonjezera 10 μ M diazepam;Jujuboside A82 μ M (pafupifupi 0.1g / L) ndi 41 μ M (pafupifupi 0.05 g / L) adawonjezedwa ku magulu a jua-h (mlingo waukulu wa Jujuboside A) ndi wa jua-l (wochepa Jujuboside A), motsatana.RNA yonse idasiyanitsidwa ndi ma cell kuti aunikenso [1].

Kuyesera kwa zinyama:mbewa: a anabayidwa kudzera mu ICV β 1-42 anapangitsa kuwonongeka kwa chidziwitso mu mbewa.Kenako, mbewa zinabayidwa ndi Jujuboside A (0.02 ndi 0.2 mg / kg) intraventricular (ICV) kwa masiku asanu.Y maze, kupewa mwachangu komanso mayeso a maze a Morris madzi adachitidwa pa mbewa [1].