Iimveliso

Igama lesiNgesi:tanshinone IIA
Igama lesiTshayina:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA umsebenzi webhayoloji

Inkcazo:
I-Tanshinone IIA (i-Tan IIA) yenye yezinto eziphambili ezinyibilikayo ezinamafutha kwiingcambu zengcambu ebomvu iSalvia miltiorrhiza.I-Tanshinone IIA inokuthintela i-angiogenesis ngokujolisa kwi-protein kinase domain ye-VEGF / VEGFR2.

Iindidi ezifanelekileyo:
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Iimveliso zeNdalo > > IiQuinones

Ekujoliswe kuko:
VEGF/VEGFR2[1]

Isifundo se-In Vitro:Iziphumo ze-antitumor ze-tanshinone IIA ziquka ukuthintela ukwanda kweeseli ze-tumor, ukuphazamisa umjikelo weseli ye-tumor, ukukhuthaza i-apoptosis ye-tumor cell kunye nokuthintela ukuhlasela kweseli ye-tumor kunye ne-metastasis.I-Tanshinone IIA yayinempembelelo ye-anti proliferative kwiiseli ze-A549: i-IC50 ye-tanshinone IIA emva kwe-24, i-48 kunye neeyure ze-72 zaziyi-145.3, i-30.95 kunye ne-11.49, ngokulandelelanayo μ M. CCK-8 assay yasetyenziselwa ukuvavanya i-tanshinone I80 μ-M80 μ-M25. Umsebenzi okhulayo weeseli ze-A549 eziphathwayo kwi-24, i-48 kunye neeyure ze-72, ngokulandelanayo.Iziphumo ze-CCK-8 zibonise ukuba i-tanshinone IIA inokuthintela kakhulu ukwanda kweeseli ze-A549 ngendlela exhomekeke kumthamo kunye nexesha elixhomekeke kwixesha.I-apoptosis ebalulekileyo kunye nokuthintela ukukhula kweeseli zeeseli ze-A549 zabonwa kwiiyure ezingama-48 emva konyango lweziyobisi (ugxininiso olusetyenzisiweyo lwalumalunga nexabiso le-IC50: tanshinone iia31 kwi-A549) μ M) . μ M) , phantsi lawula ukubonakaliswa kwe-VEGF kunye ne-VEGFR2 iprotheni kwiqela lonyango lweziyobisi kunye ne-vector [1].I-Tanshinone IIA yenye yezona zinto zininzi kakhulu zengcambu yeSalvia miltiorrhiza, enokukhusela iiseli ze-H9c2 kwi-apoptosis.Iiseli ze-H9c2 eziphathwa nge-tanshinone IIA inhibited angiotensin II yenze i-apoptosis ngokuhla ilawula intetho ye-PTEN (i-phosphatase kunye ne-tensin homologue).I-PTEN yi-tumor suppressor edlala indima ephambili kwi-apoptosis.I-Tanshinone IIA inqanda i-angiotensin II (i-AngII) - i-apoptosis eyenziwe ngokunciphisa i-phosphatase kunye ne-tensin homologue (PTEN) [2].I-Tanshinone IIA inciphisa ukubonakaliswa kweprotheni ye-EGFR, kwaye i-IGFR ivimba indlela ye-PI3K / Akt / mTOR kumhlaza wesisu se-AGS cells [3].

Uvavanyo lweSeli:Iiseli ze-A549 zibalwe kwisigaba se-logarithmic kunye neeseli ze-6000 (i-90 μ L umthamo) kwi-plate ye-96.Ngaba i-10 μ L imilinganiselo eyahlukeneyo ye-tanshinone IIA (ugxininiso lokugqibela 80,60,40,30,20,15,10,5 kunye ne-2.5 μ M) kunye ne-ADM (uxinzelelo lokugqibela 8,4,2,1,0.5 kunye ne-0.25 μ M ) Yongezwa kwiqela leziyobisi, ngelixa iqela lokulawula elibi (iqela le-carrier) longezwa kuphela i-10 μ Ldmso okanye i-saline eqhelekileyo ngaphandle kwe-tanshinone IIA okanye i-Adm Hlanganisa iiseli kunye ne-CCK-8 reagent (100 μ L / ml medium) ukuze enye iiyure ze-2, kwaye i-absorbence ifundwe kwi-450 nm usebenzisa umfundi we-microplate.Izinga lokuthintela ukwanda kweeseli libalwa ngokwefomula ilandelayo: izinga lokuthintela ukwanda (%) = 1 - [(A1-A4) / (A2-A3)] × 100, apho i-A1 ixabiso le-OD leqela lokulinga iziyobisi, I-A2 yixabiso le-OD yeqela elingenanto yokulawula, i-A3 yixabiso le-OD ye-RPMI1640 ephakathi ngaphandle kweeseli, kwaye i-A4 yixabiso le-OD yeyeza kunye ne-concentration efanayo ne-A1 kodwa ngaphandle kweeseli.Ixabiso le-IC50 libalwe ngokuhlalutya kwe-nonlinear regression analysis usebenzisa i-graphpad prism software [1], emele i-concentration yeziyobisi ebonisa i-50% inhibition yokukhula kweeseli.

Isalathiso:[1].Xie J, kunye nabanye.Impembelelo ye-antitumor ye-tanshinone IIA kwi-anti-proliferation kunye nokunciphisa inkcazo ye-VEGF / VEGFR2 kumntu ongeyena omncinci umhlaza wamaphaphu we-cell A549.Acta Pharm Sin B. 2015 Nov;5(6):554-63.
[2].UZhang Z, et al.I-Tanshinone IIA inqanda i-apoptosis kwi-myocardium ngokubangela ukubonakaliswa kwe-microRNA-152-3p kwaye ngaloo ndlela inciphisa i-PTEN.Am J Transl Res.2016 Jul 15;8(7):3124-32.
[3].Su CC, et al.I-Tanshinone IIA inciphisa i-protein expression ye-EGFR, kunye ne-IGFR ivala indlela ye-PI3K / Akt / mTOR kwi-gastric carcinoma AGS iiseli zombini kwi-vitro kunye ne-vivo.I-Oncol Rep. 2016 Aug;36(2):1173-9.