Ngwaahịa

Aha Bekee:tanshinone IIA
Utu aha Chinese:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA ọrụ ndu

Nkọwa:
Tanshinone IIA (Tan IIA) bụ otu n'ime ndị bụ isi abụba soluble mejupụtara na mgbọrọgwụ nke red mgbọrọgwụ Salvia miltiorrhiza.Tanshinone IIA nwere ike igbochi angiogenesis site na ilekwasị anya na ngalaba protein kinase nke VEGF / VEGFR2.

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Ebumnuche:
VEGF/VEGFR2[1]

N'ime Vitro Ọmụmụ:Mmetụta antitumor nke tanshinone IIA gụnyere igbochi mmụba cell tumor, na-akpaghasị usoro mkpụrụ ndụ tumor, na-akwalite apoptosis tumor cell na igbochi mbuso agha cell tumor na metastasis.Tanshinone IIA nwere mmetụta mgbochi proliferative na mkpụrụ ndụ A549: IC50 nke tanshinone IIA mgbe 24, 48 na 72 awa bụ 145.3, 30.95 na 11.49, n'otu n'otu μ M. CCK-8 nyocha iji nyochaa tanshinone IIA (2.5-80 μ M) Ọrụ mmụba nke mkpụrụ ndụ A549 a na-agwọ na awa 24, 48 na 72, n'otu n'otu.Nsonaazụ CCK-8 gosiri na tanshinone IIA nwere ike igbochi mmụba nke mkpụrụ ndụ A549 n'ụzọ na-adabere na dose na oge.Achọpụtara apoptosis dị ịrịba ama na mgbochi cell cell nke mkpụrụ ndụ A549 48 awa mgbe ọgwụgwọ ọgwụ gasịrị (ntụkwasị uche na-eji ihe dị ka IC50 uru: tanshinone iia31 na A549) μ M.) Western blotting kpughere ikpughe na tanshinone IIA (31) na sel A549 maka awa 48. μ M) , gbadaa na-ahazi okwu VEGF na VEGFR2 protein n'ime otu ọgwụgwọ ọgwụ na vector [1].Tanshinone IIA bụ otu n'ime ihe kachasị ukwuu nke mgbọrọgwụ Salvia miltiorrhiza, nke nwere ike ichebe mkpụrụ ndụ H9c2 site na apoptosis.Mkpụrụ ndụ H9c2 ejiri tanshinone IIA gbochie angiotensin II kpatara apoptosis site n'ịchịkwa okwu PTEN (phosphatase na tensin homologue).PTEN bụ ihe mgbochi akpụ nke na-arụ ọrụ dị mkpa na apoptosis.Tanshinone IIA na-egbochi angiotensin II (AngII) - apoptosis kpatara site n'ịchịkwa okwu nke phosphatase na tensin homologue (PTEN) [2].Tanshinone IIA na-ebelata okwu protein nke EGFR, na IGFR na-egbochi ụzọ PI3K / Akt / mTOR na ọrịa cancer gastric AGS cell [3].

Nnwale cell:A na-agụ mkpụrụ ndụ A549 na oge logarithmic na sel 6000 (olu 90 μ L) na efere 96 nke ọma.Ga 10 μ L iche iche nke tanshinone IIA (nchịkọta ikpeazụ 80,60,40,30,20,15,10,5 na 2.5 μ M) na ADM (nchịkọta ikpeazụ 8,4,2,1,0.5 na 0.25 μM). ) A na-agbakwunye ya na otu ọgwụ, ebe ndị na-ahụ maka njikwa na-adịghị mma (otu ndị na-ebu) na-agbakwunye naanị 10 μ Ldmso ma ọ bụ saline nkịtị na-enweghị tanshinone IIA ma ọ bụ Adm. Gwakọta sel na CCK-8 reagent (100 μ L / ml medium) maka. awa 2 ọzọ, a na-agụkwa nnabata ahụ na 450 nm site na iji onye na-agụ microplate.A na-agbakọ ọnụ ọgụgụ nkwụsị nke cell proliferation dị ka usoro ndị a si dị: ọnụ ọgụgụ mgbochi mmụba (%) = 1 - [(A1-A4) / (A2-A3)] × 100, ebe A1 bụ uru OD nke otu nnwale ọgwụ, A2 bụ uru OD nke otu njikwa oghere, A3 bụ uru OD nke RPMI1640 na-enweghị mkpụrụ ndụ, yana A4 bụ uru OD nke ọgwụ ahụ nwere otu itinye uche dị ka A1 mana enweghị sel.A gbakọrọ uru IC50 site na nyocha nkwụghachi azụ na-abụghị nke na-eji ngwanrọ graphpad prism [1], nke na-anọchite anya ntinye ọgwụ na-egosi mgbochi 50% sel.

Ntụaka:[1].Xie J, et al.Mmetụta antitumor nke tanshinone IIA na mgbochi mgbasawanye na mbelata okwu VEGF/VEGFR2 na ọrịa cancer akpa ume mmadụ A549 na-abụghị obere cell.Acta Pharm Sin B. 2015 Nov;5 (6): 554-63 .
[2].Zhang Z, et al.Tanshinone IIA na-egbochi apoptosis na myocardium site na ime ka microRNA-152-3p okwu wee si otú a na-ebelata PTEN.Am J Transl Res.2016 Jul 15;8 (7): 3124-32 .
[3].Su CC, et al.Tanshinone IIA na-ebelata okwu protein nke EGFR, yana IGFR na-egbochi ụzọ PI3K/Akt/mTOR na mkpụrụ ndụ carcinoma AGS gastric ma na vitro na na vivo.Oncol Rep. 2016 Ọgọst;36 (2): 1173-9 .