Kayayyaki

Sunan Ingilishi:tanshinone II
Laƙabin Sinawa:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA nazarin halittu

Bayani:
Tanshinone IIA (Tan IIA) yana ɗaya daga cikin manyan abubuwan da ke narkewa a cikin tushen ja tushen Salvia miltiorrhiza.Tanshinone IIA na iya hana angiogenesis ta hanyar niyya yankin kinase sunadaran VEGF / VEGFR2.

Rukunin da suka dace:
Filin bincike > > cututtukan zuciya
Samfuran Halitta >> Quinones

Manufar:
VEGF/VEGFR2[1]

A cikin Nazarin Vitro:Tantanin rashin kunshin na Tankinon Iia ya haɗa da hanzarta hanzarta yin yaduwar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, inganta ƙwayar ƙwayar ƙwayar ƙwayar cuta.Tanshinone IIA yana da tasiri mai tasiri akan kwayoyin A549: IC50 na tanshinone IIA bayan 24, 48 da 72 hours sun kasance 145.3, 30.95 da 11.49, bi da bi μ M. CCK-8 an yi amfani da gwajin tanshinone IIA (2.5-80 μ M) Ayyukan yaduwa na ƙwayoyin A549 da aka kula da su a cikin sa'o'i 24, 48 da 72, bi da bi.Sakamakon CCK-8 ya nuna cewa tanshinone IIA zai iya hana yaduwar kwayoyin A549 a cikin tsarin da aka dogara da kashi da lokaci.An lura da mahimmancin apoptosis da haɓakar haɓakar ƙwayoyin sel na sel A549 48 hours bayan jiyya na miyagun ƙwayoyi (haɗin da aka yi amfani da shi shine game da ƙimar IC50: tanshinone iia31 akan A549) μ M. μ M), saukar da daidaita maganganun VEGF da furotin VEGFR2 a cikin rukunin magungunan ƙwayoyi da vector [1].Tanshinone IIA yana daya daga cikin abubuwan da suka fi dacewa na tushen Salvia miltiorrhiza, wanda zai iya kare kwayoyin H9c2 daga apoptosis.Kwayoyin H9c2 da aka bi da su tare da tanshinone IIA sun hana angiotensin II haifar da apoptosis ta hanyar saukar da tsarin PTEN (phosphatase da tensin homologue).PTEN mai hana ƙari ne wanda ke taka muhimmiyar rawa a cikin apoptosis.Tanshinone IIA yana hana angiotensin II (AngII) - haifar da apoptosis ta hanyar daidaita yanayin phosphatase da tensin homologue (PTEN) [2].Tanshinone IIA yana rage bayanin furotin na EGFR, kuma IGFR yana toshe hanyar PI3K / Akt / mTOR a cikin ƙwayoyin AGS na ciwon daji [3].

Gwajin Kwayoyin Halitta:Kwayoyin A549 an ƙidaya su a cikin lokaci na logarithmic da sel 6000 (ƙarar 90 μ L) a cikin farantin rijiyar 96.Za 10 μ L daban-daban na tanshinone IIA (mafi girma na 80,60,40,30,20,15,10,5 da 2.5 μ M) da kuma ADM (ƙarshen 8,4,2,1,0.5 da 0.25 μM). ) An ƙara shi a cikin rukunin magunguna, yayin da ƙungiyar kulawa mara kyau (ƙungiyar masu ɗaukar hoto) an ƙara kawai 10 μ Ldmso ko saline na yau da kullun ba tare da tanshinone IIA ko Adm ba. wasu sa'o'i 2, kuma an karanta abin sha a 450 nm ta amfani da mai karanta microplate.Ana ƙididdige ƙimar hana haɓakar ƙwayoyin cuta bisa ga tsari mai zuwa: ƙimar hana haɓakar haɓakawa (%) = 1 - [(A1-A4) / (A2-A3)] × 100, inda A1 shine ƙimar OD na rukunin gwaji na miyagun ƙwayoyi, A2 shine ƙimar OD na ƙungiyar kulawa mara kyau, A3 shine ƙimar OD na RPMI1640 matsakaici ba tare da sel ba, kuma A4 shine ƙimar OD na miyagun ƙwayoyi tare da maida hankali ɗaya kamar A1 amma ba tare da sel ba.An ƙididdige ƙimar IC50 ta hanyar nazarin koma bayan da ba na kan layi ba ta amfani da software na graphpad priism [1], wanda ke wakiltar ƙaddamar da miyagun ƙwayoyi yana nuna hana haɓakar ƙwayoyin 50%.

Magana:[1].Xie J, et al.Sakamakon antitumor na tanshinone IIA akan anti-proliferation da kuma rage VEGF/VEGFR2 magana akan kwayar cutar ciwon huhu na A549 na ɗan adam.Acta Pharm Sin B. 2015 Nov;5 (6): 554-63.
[2].Zhang Z, et al.Tanshinone IIA yana hana apoptosis a cikin myocardium ta hanyar haifar da maganganun microRNA-152-3p kuma ta haka yana rage PTEN.Am J Transl Res.2016 Yuli 15;8 (7): 3124-32.
[3].Su CC, et al.Tanshinone IIA yana rage bayanin furotin na EGFR, kuma IGFR yana toshe hanyar PI3K/Akt/mTOR a cikin ƙwayoyin carcinoma AGS na ciki duka a cikin vitro da in vivo.Wakilin Oncol 2016 Agusta;36 (2): 1173-9.