Alaabta

Sharaxaad:tanshinone I waa nooca IIA aadanaha recombinant sPLA2 iyo Bakaylaha Recombinant cPLA2 inhibitor leh IC50 of 11 siday u kala horreeyaan μ M iyo 82 μM.

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Bartilmaameedka:IC50: 11 μM (sPLA2), 82 μM (cPLA2) [1].

Barashada Vitro dhexdeeda:Tanshinone I waxay joojisay samaynta PGE2 by LPS macrophages cayriin (IC50 = 38 μ M) Marka tanshinone I iyo LPS lagu daray isku mar, xaruntu waxay si weyn u joojisay 10-100 μ PGE2 wax soo saarka M (IC50 = 38 μM) Marka lagu daro ka dib soo saarista COX-2 oo dhameystiran, tanshinone waxaan sidoo kale hoos u dhigay wax soo saarka PGE2 (IC50 = 46) μ M） Waxay si toos ah u joojisaa dhaqdhaqaaqa COX-2 iyo / ama waxay saamaysaa dhaqdhaqaaqa PLA2. Markii tanshinone la igu dhejiyay laba nooc oo kala duwan oo ah fosfolipase A2 (PLA2), waxay si weyn u joojisay sPLA2 qaab ku xiran feejignaanta (IC50 = 11) μM). awoodda, tanshinone I sidoo kale waxay joojisay cPLA2 (IC50 = 82) μ M）[1]

Barashada Vivo:tanshinone Waxaan muujiyay dhaqdhaqaaqa ka-hortagga caabuqa ee carrageenan ee bararka laf-dhabarka iyo adjuvant-ku-daba-beelka arthritis-ka ee jiirka.Si loo aasaaso dhaqdhaqaaqa anti-bararka ee tanshinone I, moodooyinka xayawaanka qadiimiga ah ee caabuqa ba'an iyo kuwa daba-dheeraada [rat carrageenan (CGN) - bararka bararka iyo jiirka adjuvant ee arthritis-ka (AIA)] ayaa la isticmaalay.Marka tanshinone afka laga qaato, waxay muujisay firfircooni ka hortag ah oo ka dhan ah bararka bararka ee CGN (47% xakamaynta 160 mg / kg), halka IC50 ee indomethacin ay ahayd 7.1 mg / kg.AIA, tanshinone waxaan siiyay 27% joojinta caabuqa labaad maalinta 18 ee qiyaasta afka ee 50 mg / kg / maalin, halka prednisolone (5 mg / kg / day) ay muujisay xakameyn wax ku ool ah (65%) [1]

Tijaabada Kinase:Sida isha PLA2, recombinant sPLA2 (nooca IIA) ee bini'aadamka ayaa laga nadiifiyay unugyada CHO oo lagu beddelay hidda-wadaha PLA2, iyo bakaylaha recombinant platelet cPLA2 ayaa laga helay muujinta baculovirus.Isku darka falcelinta caadiga ah (200) μ 50) Waxa ay ka kooban tahay 100 mm Tris HCl buffer (pH 9.0), 6 mM CaCl2 iyo 20 nmol of 1-acyl - [1-14C] - arachidonyl Sn glycerol fosfate ethanolamine (2000 CPM / nmol).Ama ma jirin tanshinone I. falcelinta ayaa lagu bilaabay iyadoo lagu daray 50NG oo la safeeyey sPLA2 ama cPLA2.Ka dib 20 daqiiqo at 37 ℃, asiidhyada dufanka bilaashka ah ee la soo saaray ayaa la falanqeeyay.Xaaladahan caadiga ah, qiyaastii 10% asiidhyada dufanka bilaashka ah ayaa laga sii daayaa substrate phospholipid ee lagu daray isku darka falcelinta iyada oo aan tanshinone I [1].

Tijaabada Unug:Unugyada 264.7 cayriin ayaa lagu dhaqay DMEM oo lagu kabay 10% FBS iyo 1% antibiyootiga 37 ℃ ee ka hooseeya 5% CO2.Marka la soo koobo, unugyadu waxa lagu beeray 96 saxan oo ceel ah (2) × 10 (5 unug / ceel).Ilaa si kale loo tilmaamo, LPS (1ug/ml) iyo tanshinone waa la igu daray oo la igu shubay 24 saacadood.U fiirsashada PGE2 ee dhexdhexaadka ah ayaa lagu cabbiray iyadoo la adeegsanayo qalabka EIA ee PGE2.Si loo go'aamiyo saameynta tanshinone I ee wax soo saarka PGE 2 ka dib COX-2 induction, unugyada ayaa lagu qasi jiray LPS (1 μ G / ml) saacadaha 24 oo si fiican loo dhaqay.Kadib, tanshinone waxaa la igu daray LPS la'aan iyo unugyadu waxay ahaayeen kuwo la dhaqay 24 saacadood oo kale.Xoojinta PGE2 waxaa laga cabiray dhexdhexaadka.Baaritaanka MTT waxaa loo isticmaalay in lagu baaro cytotoxicity tanshinone I ee unugyada cayriin.Tanshinone I ee 100 μM ma muujin wax cytotoxicity ah [1].

Tijaabada Xoolaha:si loo qiimeeyo dhaqdhaqaaqa xannibaadda ee tanshinone I ee moodooyinka xayawaanka ba'an iyo kuwa daba-dheeraada, carrageenan (CGN) - bararka laf-dhabarka ee xanuunka leh iyo qaababka arthritis-ka ee adjuvant (AIA) ayaa la isticmaalay.Marka la soo koobo, 1% CGN oo lagu milmay pyrogen-free saline (0.05 ml) ayaa lagu duray garabka midig ee jiirka ee baaritaanka bararka bararka.5 saacadood ka dib, bararka cidiyaha la daweeyay ayaa lagu qiyaasay iyadoo la isticmaalayo plethysmograph.Tanshinone I ku milmay 0.5% CMC waxaa lagu maamulay afka 1 saac kahor duritaanka CGN.Tijaabada AIA, bararka arthritic waxaa sababay in lagu duro Mycobacterium lactis (0.6ml / rat) oo ku milmay saliidda macdanta oo la galiyay lugta midig ee jiirka.Tanshinone waxaa la i maamuli jiray afka maalin kasta.Balaadhinta cidiyaha la daweeyay iyo kuwa aan la daweyn ayaa lagu cabiray iyadoo la isticmaalayo plethysmograph.

Tixraacyo:[1] Kim SY, iyo al.Saamaynta Tanshinone I ee ka go'doonsan Salvia miltiorrhiza bunge ee dheef-shiid kiimikaadka aashitada arachidonic iyo jawaabaha caabuqa ee vivo.Phytother Res.2002 Nof;16 (7): 616-20.